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논문 기본 정보

자료유형
학술저널
저자정보
Hur, Yong-Soo (Maria Fertility Hospital) Park, Jeong-Hyun (Department of Anatomy, Kangwon National University School of Medicine) Ryu, Eun-Kyung (Maria Fertility Hospital) Yoon, Hae-Jin (Maria Fertility Hospital) Yoon, San-Hyun (Fertility Research Center) Hur, Chang-Young (Maria Fertility Hospital) Lee, Won-Don (Maria Fertility Hospital) Lim, Jin-Ho (Maria Fertility Hospital)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제38권 제2호
발행연도
2011.1
수록면
87 - 92 (6page)

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Objective: This study aimed to determine the safety and clinical effect of artificial shrinkage (AS) in terms of assisted hatching of fresh blastocysts. Also, we evaluated the correlation between patient age and the effect of AS on clinical outcome. Methods: Two AS methods, using a 29-gauge needle and laser pulse, were compared. Seventy-three blastocysts were shrunk using a 29-gauge needle and the same number of other blastocysts were shrunk by a laser pulse. We evaluated the shrunken blastocysts hourly and considered them viable if they re-expanded >70%. Blastocyst transfer cycles (n=134) were divided into two groups: a control group consisted of the cycles whose intact embryos were transferred (n=100), while the AS group consisted of the cycles whose embryos were replaced following AS (n=34). The implantation and pregnancy rates of the control group and AS group were compared ($p$ <0.05). Results: The re-expansion rates of the 29-gauge needle and laser pulse AS groups were similar (56 [76.7%] vs. 62 [84.9%], respectively). All of the remaining shrunken blastocysts were re-expanded within 2 hours. There was no degeneration of shrunken blastocysts. The total and clinical pregnancy rate of the AS group (23 [67.6%]; 20 [58.8%], respectively) was significantly higher than that of the control group (47 [47.0%]; 39 [39.0%], respectively). In the older patient group, there was no difference in the clinical outcomes between the AS and control groups. Conclusion: These results suggest that AS of blastocoele cavity, followed by the transfer, would be a useful approach to improve the clinical outcome in cycles in which fresh blastocyst stage embryos are transferred.
#Artificial Shrinkage #Embryo Transfer #Assisted Hatching #In Vitro Fertilization #Human

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참고문헌 (33)

참고문헌 신청
Houghton FD / 2003 / Na+, K+, ATPase activity in the human and bovine preimplantation embryo / Dev Biol 263:360~366 google schola Biggers JD / 1998 / Reflections on the culture of the preimplantation embryo / Int J Dev Biol 42:897~884 google schola Watson AJ / 2001 / Regulation of blastocyst formation / Front Biosci 6:708~730 google schola Veeck LL / 2003 / An atlas of human blastocysts / Parthenon Pub. Group google schola Glass RH / 1973 / Mouse blastocyst re-expansion following puncture and treatment with inhibitors / J Reprod Fertil 35:533~536 google schola

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