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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제41권 제3호
- 발행연도
- 2014.1
- 수록면
- 115 - 119 (5page)
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초록· 키워드
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Objective: This study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution. Methods: Mouse two-cell embryos were collected and cultured to blastocysts. Two vitrification solutions were prepared. The control solution was composed of 25% glycerol, 25% ethylene glycol, and 0.5 M sucrose (G25E250.5S) containing 2.5 mL glycerol, 2.5 mL ethylene glycol, 2 mL SSS, and 0.855 g sucrose in 5 mL PB1. The experimental solution was composed of 25% glycerol and 25% ethylene glycol (G25E25) and contained 2.5 mL glycerol and 2.5 mL ethylene glycol in 5 mL PB1. Artificial shrinkage was conducted by aspirating the blastocoelic fluid using an ICSI pipette. To examine the effect of sucrose in the vitrification solution on the survival rate of mouse blastocysts, the shrunken-equilibrated blastocysts were rehydrated or vitrified after being exposed to one of the two vitrification solutions. After exposure and the vitrification-thawing process, the re-expansion rate and hatching rate were evaluated after 6 hours of in vitro culture. Results: The re-expansion rate of mouse blastocysts exposed to vitrification solution with and without sucrose were not different in the experimental solution (without sucrose) (98%) and the control solution (with sucrose) (92%) (p>0.05). The hatching rate was higher in the experimental solution (95%) than in the control solution (88%), but did not differ across two treatments (p>0.05). The re-expansion rate of mouse blastocysts vitrified in the control solution was 92% and 94%, respectively (p>0.05), and the hatching rate was higher in the experimental solution (90%) than in the control solution (74%) (p<0.05). Conclusion: Sucrose need not be added in vitrification solution for freezing of artificially shrunken mouse blastocysts.
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참고문헌 (39)
참고문헌 신청
Gardner DK / 1998 / A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization / Hum Reprod 13:3434~3440
Jones GM / 1998 / Evolution of a culture protocol for successful blastocyst development and pregnancy / Hum Reprod 13:169~177
Menezo Y / 1992 / Freezing cocultured human blastocysts / Fertil Steril 58:977~980
Bonsi A / 1995 / Blastocyst transfer and freezing: can this help us to improve the success of assisted reproduction? / Singapore J Obstet Gynecol 26:13~17
Kaufman RA / 1995 / Cocultured blastocyst cryopreservation: experience of more than 500 transfer cycles / Fertil Steril 64:1125~1129
Jones GM / 1998 / Evolution of a culture protocol for successful blastocyst development and pregnancy / Hum Reprod 13:169~177
Menezo Y / 1992 / Freezing cocultured human blastocysts / Fertil Steril 58:977~980
Bonsi A / 1995 / Blastocyst transfer and freezing: can this help us to improve the success of assisted reproduction? / Singapore J Obstet Gynecol 26:13~17
Kaufman RA / 1995 / Cocultured blastocyst cryopreservation: experience of more than 500 transfer cycles / Fertil Steril 64:1125~1129
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