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논문 기본 정보

자료유형
학술저널
저자정보
Shin, Hyejin (Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University) Choi, Soyoung (Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University) Lim, Hyunjung Jade (Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제41권 제3호
발행연도
2014.1
수록면
125 - 131 (7page)

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Objective: Under estrogen deficiency, blastocysts cannot initiate implantation and enter dormancy. Dormant blastocysts live longer in utero than normal blastocysts, and autophagy has been suggested as a mechanism underlying the sustained survival of dormant blastocysts during delayed implantation. Autophagy is a cellular degradation pathway and a central component of the integrated stress response. Reactive oxygen species (ROS) are produced within cells during normal metabolism, but their levels increase dramatically under stressful conditions. We investigated whether heightened autophagy in dormant blastocysts is associated with the increased oxidative stress under the unfavorable condition of delayed implantation. Methods: To visualize ROS production, day 8 (short-term dormancy) and day 20 (long-term dormancy) dormant blastocysts were loaded with $1-{\mu}M$ 5-(and-6)-chloromethyl-2', 7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-$H_2DCFDA$). To block autophagic activation, 3-methyladenine (3-MA) and wortmannin were used in vivo and in vitro, respectively. Results: We observed that ROS production was not significantly affected by the status of dormancy; in other words, both dormant and activated blastocysts showed high levels of ROS. However, ROS production was higher in the dormant blastocysts of the long-term dormancy group than in those of the short-term group. The addition of wortmannin to dormant blastocysts in vitro and 3-MA injection in vivo significantly increased ROS production in the short-term dormant blastocysts. In the long-term dormant blastocysts, ROS levels were not significantly affected by the treatment of the autophagy inhibitor. Conclusion: During delayed implantation, heightened autophagy in dormant blastocysts may be operative as a potential mechanism to reduce oxidative stress. Further, ROS may be one of the potential causes of compromised developmental competence of long-term dormant blastocysts after implantation.
#Autophagy #Delayed implantation #Dormant blastocyst #Reactive oxygen species

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참고문헌 (35)

참고문헌 신청
Lopes FL / 2004 / Embryonic diapause and its regulation / Reproduction 128:669~678 google schola Murphy BD / 2012 / Embryonic diapause: advances in understanding the enigma of seasonal delayed implantation / Reprod Domest Anim 47(Suppl 6):121~124 google schola Renfree MB / 1981 / Embryonic diapause in marsupials / J Reprod Fertil Suppl 29:67~78 google schola Renfree MB / 2000 / Diapause / Annu Rev Physiol 62:353~375 google schola Kondoh E / 2009 / Stress affects uterine receptivity through an ovarian-independent pathway / Hum Reprod 24:945~953 google schola

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