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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Yunna (Department of Biomedical Science, College of Life Science, CHA University) Kim, Eun-Young (Department of Biomedical Science, College of Life Science, CHA University) Seo, You-Mi (Department of Biomedical Science, College of Life Science, CHA University) Yoon, Tae Ki (CHA Research Institute, Fertility Center, CHA General Hospital) Lee, Woo-Sik (CHA Research Institute, Fertility Center, CHA General Hospital) Lee, Kyung-Ah (Department of Biomedical Science, College of Life Science, CHA University)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제39권 제2호
발행연도
2012.1
수록면
58 - 67 (10page)

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Objective: Previously, we identified that transketolase (Tkt), an important enzyme in the pentose phosphate pathway, is highly expressed at 2 hours of spontaneous maturation in oocytes. Therefore, this study was performed to determine the function of Tkt in meiotic cell cycle regulation, especially at the point of germinal vesicle breakdown (GVBD). Methods: We evaluated the loss-of-function of Tkt by microinjecting Tkt double-stranded RNAs (dsRNAs) into germinal vesicle-stage oocytes, and the oocytes were cultured in vitro to evaluate phenotypic changes during oocyte maturation. In addition to maturation rates, meiotic spindle and chromosome rearrangements, and changes in expression of other enzymes in the pentose phosphate pathway were determined after Tkt RNA interference (RNAi). Results: Despite the complete and specific knockdown of Tkt expression, GVBD occurred and meiosis was arrested at the metaphase I (MI) stage. The arrested oocytes exhibited spindle loss, chromosomal aggregation, and declined maturation promoting factor and mitogen-activated protein kinase activities. The modified expression of two enzymes in the pentose phosphate pathway, Prps1 and Rbks, after Tkt RNAi and decreased maturation rates were amended when ribose-5-phosphate was supplemented in the culture medium, suggesting that the Tkt and pentose phosphate pathway are important for the maturation process. Conclusion: We concluded that Tkt and its associated pentose phosphate pathway play an important role in the MI-MII transition of the oocytes' meiotic cell cycle, but not in the process of GVBD.
#Oocyte #Meiosis #RNA interference #Transketolase #Pentose phosphate pathway

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참고문헌 (28)

참고문헌 신청
Pincus G / 1935 / The comparative behavior of mammalian dggs in vivo and in vitro: I. the activation of ovarian eggs / J Exp Med 62:665~675 google schola Schmitt A / 2002 / Signalling pathways in oocyte meiotic maturation / J Cell Sci 115:2457~2459 google schola Comizzoli P / 2003 / Up-regulation of glucose metabolism during male pronucleus formation determines the early onset of the s phase in bovine zygotes / Biol Reprod 68:1934~1940 google schola Downs SM / 1998 / Meiotic induction in cumulus cell-enclosed mouse oocytes: involvement of the pentose phosphate pathway / Biol Reprod 58:1084~1094 google schola Urner F / 2005 / Involvement of the pentose phosphate pathway and redox regulation in fertilization in the mouse / Mol Reprod Dev 70:494~503 google schola

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