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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 321 - 328 (8page)
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초록· 키워드
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The oily bittering Acheilognathus koreensis is a freshwater species that is endemic to Korea and is experiencing severe declines in natural populations as a result of habitat fragmentation and water pollution. For the conservation and restoration of this species, it is necessary to assess its genetic diversity at the population level. We developed 13 polymorphic microsatellite loci that were used to analyze the genetic diversity of two populations collected from the Kum River and the Tamjin River in Korea. All loci exhibited Mendelian inheritance patterns when examined in controlled crosses. Both populations revealed high levels of variability, with the number of alleles ranging from 3 to 20 and observed and expected heterozygosities ranging from 0.500 to 0.969 and from 0.529 to 0.938, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium, and one pair of loci showed significant linkage disequilibrium after Bonferroni correction. Pairwise $F_{ST}$ and genetic distance estimation showed significant differences between two populations. These results suggest that the microsatellites developed herein can be used to study the genetic diversity, population structure and conservation measure of A. koreensis.
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참고문헌 (39)
참고문헌 신청
안동하 / 2011 / Isolation and characterization of microsatellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis / Animal Cells and Systems 15(1):37~43
Alghanim HJ / 2003 / Development of microsatellite markers in Cannabis sativa for DNA typing and genetic relatedness analyses / Anal Bioanal Chem 376:1225~1233
안혜숙 / 2010 / Ten new microsatellite markers in cutlassfish Trichiurus lepturus derived from an enriched genomic library / Animal Cells and Systems 14(3):169~174
Arora R / 2004 / Genetic diversity analysis of two buffalo populationsof northern India using microsatellite markers / J Anim Breed Genet 121:111~118
Asahida T / 1996 / Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea / Fisheries Sci 62:727~730
Alghanim HJ / 2003 / Development of microsatellite markers in Cannabis sativa for DNA typing and genetic relatedness analyses / Anal Bioanal Chem 376:1225~1233
안혜숙 / 2010 / Ten new microsatellite markers in cutlassfish Trichiurus lepturus derived from an enriched genomic library / Animal Cells and Systems 14(3):169~174
Arora R / 2004 / Genetic diversity analysis of two buffalo populationsof northern India using microsatellite markers / J Anim Breed Genet 121:111~118
Asahida T / 1996 / Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea / Fisheries Sci 62:727~730
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