지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 207 - 214 (8page)
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초록· 키워드
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Burns are one of the most devastating forms of trauma and wound healing is a complex and multicellular process, which is executed and regulated by signaling networks involving numerous growth factors, cytokines, and chemokines. Recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) was specifically produced from rice cell culture through use of a recombinant technique in our laboratory. The effect of rhGM-CSF on promotion of deep second-degree burn wound healing on the back skin of a hamster model was evaluated through a randomized and double-blind trial. As macroscopic results, hamster skins of the experimental groups showed earlier recovery by new epidermis than the control groups. Immunohistochemical reactions of proliferating cell nuclear antigen and transforming growth factor-b1, which are indicators of cell proliferation, were more active in the experimental group, compared with the control group. On electron microscopy, basal cells in the epidermis of the experimental group showed oval nuclei, prominent nucleoli, numerous mitochondria and abundant free ribosomes. In addition, fibroblasts contained well-developed rough endoplasmic reticulum with dilated cisternae. Bundles of collagen fibrils filled the extracellular spaces. Particularly, ultrastructural features indicating active metabolism for regeneration of injured skin at 15 days after burn injury, including abundant euchromatin, plentiful free ribosomes, and numerous mitochondria, were observed. These findings suggest that use of rhGM-CSF could result in accelerated deep second-degree burn wound healing in animal models.
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참고문헌 (18)
참고문헌 신청
Arellano M / 2008 / Clinical uses of GM-CSF, a critical appraisal and update / Biologics 2:13~27
Barreda DR / 2004 / Regulation of myeloid development and function by colony stimulating factors / Dev Comp Immunol 28:509~554
Barrientos S / 2008 / Growth factors and cytokines in wound healing / Wound Repair Regen 16:585~601
Braunstein S / 1994 / GM-CSF activates regenerative epidermal growth and stimulates keratinocyte proliferation in human skin in vivo / J Invest Dermatol 103:601~604
Cianfaran F / 2006 / Granulocyte/ macrophage colony-stimulating factor treatment of human chronic ulcers promotes angiogenesis associated with de novo vascular endothelial growth factor transcription in the ulcer bed / Br J Dermatol 154:34~41
Barreda DR / 2004 / Regulation of myeloid development and function by colony stimulating factors / Dev Comp Immunol 28:509~554
Barrientos S / 2008 / Growth factors and cytokines in wound healing / Wound Repair Regen 16:585~601
Braunstein S / 1994 / GM-CSF activates regenerative epidermal growth and stimulates keratinocyte proliferation in human skin in vivo / J Invest Dermatol 103:601~604
Cianfaran F / 2006 / Granulocyte/ macrophage colony-stimulating factor treatment of human chronic ulcers promotes angiogenesis associated with de novo vascular endothelial growth factor transcription in the ulcer bed / Br J Dermatol 154:34~41
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