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논문 기본 정보

자료유형
학술저널
저자정보
Ahn, Dong-Ha (Department of Bivlogical Sciences, Inha University) Park, Mi-Hyun (Department of Bivlogical Sciences, Inha University) Jung, Jae-Ho (Department of Bivlogical Sciences, Inha University) Oh, Mi-Jin (Department of Biological Science, Seoul National University) Kim, Sang-Hee (Korea Polar Research Institute) Jung, Jong-Woo (Department of Science Education, Ewha Womans University) Min, Gi-Sik (Department of Bivlogical Sciences, Inha University)
저널정보
한국통합생물학회 Animal cells and systems Animal cells and systems 제15권 제1호
발행연도
2011.1
수록면
37 - 43 (7page)

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The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight micro satellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These micro satellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans.

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