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자료유형
학술저널
저자정보
Han, Jik-Hyon (School of Life Sciences and Biotechnology, Korea University) Lee, Ji-Sook (Department of Biology, Daejeon University) Lee, Chang-Seok (School of Life Sciences and Biotechnology, Korea University) Koh, Sang-Kyun (Department of Microbiology and Biotechnology, Daejeon University) Seo, Sook-Jae (Division of Life Science, Gyeongsang University) Yun, Chi-Young (Department of Biology, Daejeon University)
저널정보
한국통합생물학회 Animal cells and systems Animal cells and systems 제13권 제3호
발행연도
2009.1
수록면
297 - 304 (8page)

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A complete mRNA sequence of transferrin from the wax moth, Galleria mellonella, was obtained, and compared with those of other species. We previously reported that the sequence was most similar to those of Manduca sexta and Bombyx mori. As in other moths, G. mellonella transferrin had only one iron-binding site at its N-terminal region. Semi-qRT PCR was conducted to investigate tissue-specific distribution and transcriptional regulation of the wax moth transferrin mRNA. Larval muscle and fat body contained larger quantity of mRNA than other tested tissues. In this study, it was observed that iron and cadmium regulated transferrin transcription, and this regulation pattern was tissue specific. Iron up-regulated transferrin mRNA level in fat body, while suppressed it in the Malpighian tubules and silk glands. Cadmium decreased the mRNA level in fat body, muscle, and Malpighian tubules, but significantly increased the mRNA level in silk glands. In addition, the mRNA expression was induced by all tested pathogen-associated molecular patterns (PAMPs) including LPS, lipoteichoic acid (LTA), glucan, and even chitin.

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