Potential Biomarker of L-type Amino Acid Transporter 1 in Breast Cancer Progression

Liang, Zhongxing; Cho, Heidi T.; Williams, Larry; Zhu, Aizhi; Liang, Ke; Huang, Ke; Wu, Hui; Jiang, Chunsu; Hong, Samuel; Crowe, Ronald; Goodman, Mark M.; Shim, Hyun-Suk

추천

검색

자료유형: 학술저널

저자정보: Liang, Zhongxing (Department of Radiology, Emory University School of Medicine) Cho, Heidi T. (Department of Radiology, Emory University School of Medicine) Williams, Larry (Department of Radiology, Emory University School of Medicine) Zhu, Aizhi (Department of Radiology, Emory University School of Medicine) Liang, Ke (Department of Radiology, Emory University School of Medicine) Huang, Ke (Department of Radiology, Emory University School of Medicine) Wu, Hui (Department of Radiology, Emory University School of Medicine) Jiang, Chunsu (Department of Radiology, Emory University School of Medicine) Hong, Samuel (Department of Radiology, Emory University School of Medicine) Crowe, Ronald (Department of Radiology, Emory University School of Medicine) Goodman, Mark M. (Department of Radiology, Emory University School of Medicine) Shim, Hyun-Suk (Department of Radiology, Emory University School of Medicine)

저널정보: 대한핵의학회 Nuclear medicine and molecular imaging : NMMI Nuclear medicine and molecular imaging : NMMI 제45권 제2호

발행연도: 2011.1

수록면: 93 - 102 (10page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Purpose L-type amino acid transporter 1 (LAT1) is essential for the transport of large neutral amino acids. However, its role in breast cancer growth remains largely unknown. The purpose of the study is to investigate whether LAT1 is a potential biomarker for the diagnosis and treatment of breast cancer. Methods LAT1 mRNA and protein levels in breast cancer cell lines and tissues were analyzed. In addition, the effects of targeting LAT1 for the inhibition of breast cancer cell tumorigenesis were assessed with soft agar assay. The imaging of xenograft with anti-1-amino-3-[$^{18}F$]fluorocyclobutane-1-carboxylic acid (anti-[$^{18}F$]FACBC) PET was assessed for its diagnostic biomarker potential. Results Normal breast tissue or low malignant cell lines expressed low levels of LAT1 mRNA and protein, while highly malignant cancer cell lines and high-grade breast cancer tissue expressed high levels of LAT1. In addition, higher expression levels of LAT1 in breast cancer tissues were consistent with advanced-stage breast cancer. Furthermore, the blockade of LAT1 with its inhibitor, 2-aminobicyclo[ 2.2.1]heptane-2-carboxylic acid (BCH), or the knockdown of LAT1 with siRNA, inhibited proliferation and tumorigenesis of breast cancer cells. A leucine analog, anti-[$^{18}F$]FACBC, has been demonstrated to be an excellent PET tracer for the non-invasive imaging of malignant breast cancer using an orthotopic animal model. Conclusions The overexpression of LAT1 is required for the progression of breast cancer. LAT1 represents a potential biomarker for therapy and diagnosis of breast cancer. Anti-[$^{18}F$]FACBC that correlates with LAT1 function is a potential PET tracer for malignant breast tumor imaging.

#LAT1 #PET #Breast cancer

참고문헌 (52)

참고문헌 신청

Edwards BK / 2010 / Annual report to the nation on the status of cancer, 1975–2006, featuring colorectal cancer trends and impact of interventions (risk factors, screening, and treatment) to reduce future rates / Cancer 116:544~573

Coughlin SS / 2009 / Breast cancer as a global health concern / Cancer Epidemiol 33:315~318

Parkin DM / 2005 / Global cancer statistics, 2002 / CA Cancer J Clin 55:74~108

Anderson BO / 2008 / Guideline implementation for breast healthcare in lowincome and middle-income countries: overview of the Breast Health Global Initiative Global Summit 2007 / Cancer 113:2221~2243

Porter P / 2008 / “Westernizing” women's risks? Breast cancer in lowerincome countries / N Engl J Med 358:213~216