Curcumin Stimulates Proliferation of Spinal Cord Neural Progenitor Cells via a Mitogen-Activated Protein Kinase Signaling Pathway

Son, Sihoon; Kim, Kyoung-Tae; Cho, Dae-Chul; Kim, Hye-Jeong; Sung, Joo-Kyung; Bae, Jae-Sung

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자료유형: 학술저널

저자정보: Son, Sihoon (Department of Neurosurgery, Kyungpook National University Hospital) Kim, Kyoung-Tae (Department of Neurosurgery, Kyungpook National University Hospital) Cho, Dae-Chul (Department of Neurosurgery, Kyungpook National University Hospital) Kim, Hye-Jeong (Department of Neurosurgery, Kyungpook National University Hospital) Sung, Joo-Kyung (Department of Neurosurgery, Kyungpook National University Hospital) Bae, Jae-Sung (Department of Physiology, School of Medicine, Kyungpook National University)

저널정보: 대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제56권 제1호

발행연도: 2014.1

수록면: 1 - 4 (4page)

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Objective : The aims of our study are to evaluate the effect of curcumin on spinal cord neural progenitor cell (SC-NPC) proliferation and to clarify the mechanisms of mitogen-activated protein (MAP) kinase signaling pathways in SC-NPCs. Methods : We established cultures of SC-NPCs, extracted from the spinal cord of Sprague-Dawley rats weighing 250 g to 350 g. We measured proliferation rates of SC-NPCs after curcumin treatment at different dosage. The immuno-blotting method was used to evaluate the MAP kinase signaling protein that contains extracellular signal-regulated kinases (ERKs), p38, c-Jun $NH_2$-terminal kinases (JNKs) and ${\beta}$-actin as the control group. Results : Curcumin has a biphasic effect on SC-NPC proliferation. Lower dosage (0.1, 0.5, $1{\mu}M$) of curcumin increased SC-NPC proliferation. However, higher dosage decreased SC-NPC proliferation. Also, curcumin stimulates proliferation of SC-NPCs via the MAP kinase signaling pathway, especially involving the p-ERK and p-38 protein. The p-ERK protein and p38 protein levels varied depending on curcumin dosage (0.5 and $1{\mu}M$, p<0.05). Conclusion : Curcumin can stimulate proliferation of SC-NPCs via ERKs and the p38 signaling pathway in low concentrations.

#Curcumin #Spinal cord neural progenitor cell #Mitogen activated protein kinase

참고문헌 (32)

참고문헌 신청

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