지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 131 - 135 (5page)
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Objective : With the growing interests of bacteria as a targeting vector for cancer treatment, diverse genetically engineered Salmonella has been reported to be capable of targeting primary or metastatic tumor regions after intravenous injection into mouse tumor models. The purpose of this study was to investigate the capability of the genetically engineered Salmonella typhimurium (S. typhimurium) to access the glioma xenograft, which was monitored in mouse brain tumor models using optical bioluminescence imaging technique. Methods : U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. After tumor formation, attenuated S. typhimurium expressing bacterial luciferase (Lux) was injected into the tail vein. Bioluminescence signals from transfected cells or bacteria were monitored using a cooled charge-coupled device camera to identify the tumor location or to trace the bacterial migration. Immunofluorescence staining was also performed in frozen sections of mouse glioma xenograft. Results : The injected S. typhimurium exclusively localized in the glioma xenograft region of U87-MG-bearing mouse. Immunofluorescence staining also demonstrated the accumulation of S. typhimurium in the brain tumors. Conclusion : The present study demonstrated that S. typhimurium can target glioma xenograft, and may provide a potentially therapeutic probe for glioma.
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참고문헌 (14)
참고문헌 신청
Dinca EB / 2010 / Bioluminescence imaging of invasive intracranial xenografts : implications for translational research and targeted therapeutics of brain tumors / Neurosurg Rev 33:385~394
Hoffman S / 2006 / Temporal trends in incidence of primary brain tumors in the United States, 1985-1999 / Neuro Oncol 8:27~37
Min JJ / 2008 / Noninvasive real-time imaging of tumors and metastases using tumor-targeting light-emitting Escherichia coli / Mol Imaging Biol 10:54~61
Min JJ / 2008 / Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals / Nat Protoc 3:629~636
Momiyama M / 2012 / Inhibition and eradication of human glioma with tumor-targeting Salmonella typhimurium in an orthotopic nude-mouse model / Cell Cycle 11:628~632
Hoffman S / 2006 / Temporal trends in incidence of primary brain tumors in the United States, 1985-1999 / Neuro Oncol 8:27~37
Min JJ / 2008 / Noninvasive real-time imaging of tumors and metastases using tumor-targeting light-emitting Escherichia coli / Mol Imaging Biol 10:54~61
Min JJ / 2008 / Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals / Nat Protoc 3:629~636
Momiyama M / 2012 / Inhibition and eradication of human glioma with tumor-targeting Salmonella typhimurium in an orthotopic nude-mouse model / Cell Cycle 11:628~632
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