Valproic Acid Increases Expression of Neuronal Stem/Progenitor Cell in Spinal Cord Injury

Bang, Woo-Seok; Kim, Kyoung-Tae; Cho, Dae-Chul; Kim, Hye-Jeong; Sung, Joo-Kyung

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자료유형: 학술저널

저자정보: Bang, Woo-Seok (Department of Neurosurgery, Kyungpook National University Hospital) Kim, Kyoung-Tae (Department of Neurosurgery, Kyungpook National University Hospital) Cho, Dae-Chul (Department of Neurosurgery, Kyungpook National University Hospital) Kim, Hye-Jeong (Department of Neurosurgery, Kyungpook National University Hospital) Sung, Joo-Kyung (Department of Neurosurgery, Kyungpook National University Hospital)

저널정보: 대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제54권 제1호

발행연도: 2013.1

수록면: 8 - 13 (6page)

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Objective : This study investigates the effect of valproic acid (VPA) on expression of neural stem/progenitor cells (NSPCs) in a rat spinal cord injury (SCI) model. Methods : Adult male rats (n=24) were randomly and blindly allocated into three groups. Laminectomy at T9 was performed in all three groups. In group 1 (sham), only laminectomy was performed. In group 2 (SCI-VPA), the animals received a dose of 200 mg/kg of VPA. In group 3 (SCI-saline), animals received 1.0 mL of the saline vehicle solution. A modified aneurysm clip with a closing force of 30 grams was applied extradurally around the spinal cord at T9, and then rapidly released with cord compression persisting for 2 minutes. The rats were sacrificed and the spinal cord were collected one week after SCI. Immunohistochemistry (IHC) and western blotting sample were obtained from 5 mm rostral region to the lesion and prepared. We analyzed the nestin immunoreactivity from the white matter of ventral cord and the ependyma of central canal. Nestin and SOX2 were used for markers for NSPCs and analyzed by IHC and western blotting, respectively. Results : Nestin and SOX2 were expressed significantly in the SCI groups but not in the sham group. Comparing SCI groups, nestin and SOX2 expression were much stronger in SCI-VPA group than in SCI-saline group. Conclusion : Nestin and SOX2 as markers for NSPCs showed increased expression in SCI-VPA group in comparison with SCI-saline group. This result suggests VPA increases expression of spinal NSPCs in SCI.

#Neural stem/progenitor cell #Spinal cord injury #Valproic acid #Nestin #SOX2

참고문헌 (35)

참고문헌 신청

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