Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-${kappa}B$ in Acute Transection Spinal Cord Injury in Rats

Shrestha, Rajiv Prasad; Qiao, Jian Min; Shen, Fu Guo; Bista, Krishna Bahadur; Zhao, Zhong Nan; Yang, Jianhua

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자료유형: 학술저널

저자정보: Shrestha, Rajiv Prasad (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) Qiao, Jian Min (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) Shen, Fu Guo (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) Bista, Krishna Bahadur (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) Zhao, Zhong Nan (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) Yang, Jianhua (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University)

저널정보: 대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제56권 제5호

발행연도: 2014.1

수록면: 375 - 382 (8page)

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Objective : To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) in spinal cord injury (SCI) in rats. Methods : BMMNCs were isolated from tibia and femur by a density gradient centrifugation. After establishment of acute transection SCI, rats were divided into experiment (BMMNCs), experiment control (0.1 M PBS infused) and sham surgery groups (laminectomy without any SCI). Locomotor function was assessed weekly for 5 weeks post-injury using BBB locomotor score and urinary bladder function daily for 4 weeks post-injury. Activity of NF-${\kappa}B$ in spinal cord was assessed by immunohistochemistry and reverse transcriptase polymerase chain reaction. Results : At each time point post-injury, sham surgery group had significantly higher Basso, Beattie, Bresnahan locomotor and urinary bladder function scores than experiment and experiment control group (p<0.05). At subsequent time interval there were gradual improvement in both experiment and experiment control group, but experiment group had higher score in comparison to experiment control group (p<0.05). Comparisons were also made for expression of activated NF-${\kappa}B$ positive cells and level of NF-${\kappa}B$ messenger RNA in spinal cord at various time points between the groups. Activated NF-${\kappa}B$ immunoreactivity and level of NF-${\kappa}B$ mRNA expression were significantly higher in control group in comparison to experiment and sham surgery group (p<0.05). Conclusion : BMMNCs transplantation attenuates the expression of NF-${\kappa}B$ in injured spinal cord tissue and thus helps in recovery of neurological function in rat models with SCI.

#Cell transplantation #Inflammation #Mononuclear cells #Neuroprotective agent #NF- ${kappa}B$ #Spinal cord injury

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