Engraftment of Human Mesenchymal Stem Cells in a Rat Photothrombotic Cerebral Infarction Model : Comparison of Intra-Arterial and Intravenous Infusion Using MRI and Histological Analysis

Byun, Jun Soo; Kwak, Byung Kook; Kim, Jae Kyun; Jung, Jisung; Ha, Bon Chul; Park, Serah

추천

검색

자료유형: 학술저널

저자정보: Byun, Jun Soo (Department of Radiology, Chung-Ang University College of Medicine) Kwak, Byung Kook (Department of Radiology, Chung-Ang University College of Medicine) Kim, Jae Kyun (Department of Radiology, Chung-Ang University College of Medicine) Jung, Jisung (Department of Radiology, Chung-Ang University College of Medicine) Ha, Bon Chul (Department of Radiology, Chung-Ang University College of Medicine) Park, Serah (Department of Radiology, Chung-Ang University College of Medicine)

저널정보: 대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제54권 제6호

발행연도: 2013.1

수록면: 467 - 476 (10page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Objective : This study aimed to evaluate the hypotheses that administration routes [intra-arterial (IA) vs. intravenous (IV)] affect the early stage migration of transplanted human bone marrow-derived mesenchymal stem cells (hBM-MSCs) in acute brain infarction. Methods : Male Sprague-Dawley rats (n=40) were subjected to photothrombotic infarction. Three days after photothrombotic infarction, rats were randomly allocated to one of four experimental groups [IA group : n=12, IV group : n=12, superparamagnetic iron oxide (SPIO) group : n=8, control group : n=8]. All groups were subdivided into 1, 6, 24, and 48 hours groups according to time point of sacrifice. Magnetic resonance imaging (MRI) consisting of T2 weighted image (T2WI), $T2^*$ weighted image ($T2^*WI$), susceptibility weighted image (SWI), and diffusion weighted image of rat brain were obtained prior to and at 1, 6, 24, and 48 hours post-implantation. After final MRI, rats were sacrificed and grafted cells were analyzed in brain and lung specimen using Prussian blue and immunohistochemical staining. Results : Grafted cells appeared as dark signal intensity regions at the peri-lesional zone. In IA group, dark signals in peri-lesional zone were more prominent compared with IV group. SWI showed largest dark signal followed by $T2^*WI$ and T2WI in both IA and IV groups. On Prussian blue staining, IA administration showed substantially increased migration and a large number of transplanted hBM-MSCs in the target brain than IV administration. The Prussian blue-positive cells were not detected in SPIO and control groups. Conclusion : In a rat photothrombotic model of ischemic stroke, selective IA administration of human mesenchymal stem cells is more effective than IV administration. MRI and histological analyses revealed the time course of cell migration, and the numbers and distribution of hBM-MSCs delivered into the brain.

#MRI #Mesenchymal stem cell #Photothrombotic cerebral infarction #Superparamagnetic iron oxide #Intravascular

참고문헌 (67)

참고문헌 신청

Abdallah BM / 2005 / Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite [corrected] extensive proliferation / Biochem Biophys Res Commun 326:527~538

Akter M / 2007 / Detection of hemorrhagic hypointense foci in the brain on susceptibilityweighted imaging clinical and phantom studies / Acad Radiol 14:1011~1019

Andres RH / 2011 / The CCR2/CCL2 interaction mediates the transendothelial recruitment of intravascularly delivered neural stem cells to the ischemic brain / Stroke 42:2923~2931

Andres RH / 2008 / Potential of adult neural stem cells in stroke therapy / Regen Med 3:893~905

Arai T / 2006 / Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 t / Magn Reson Med 55:203~209