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논문 기본 정보

자료유형
학술저널
저자정보
Jin, Eun-Sun (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) Min, Joongkee (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) Jeon, Sang Ryong (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) Choi, Kyoung Hyo (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) Jeong, Je Hoon (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan)
저널정보
대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제53권 제4호
발행연도
2013.1
수록면
207 - 212 (6page)

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Objective : Recent studies have shown encouraging progress toward the use of autogenic and allogenic mesenchymal stem cells (MSCs) to arrest, or even lead to partial regeneration in, intervertebral disc (IVD) degeneration. However, this technology is still in its infancy, and further development is required. The aim of this study was to analyze whether rat adipose-derived mesenchymal stem cells (ADMSC) can differentiate towards IVD-like cells after treatment with transforming growth factor ${\beta}3$ (TGF-${\beta}3$) in vitro. We also performed quantitative analysis of gene expression for ADMSC only, ADMSCs treated with TGF-${\beta}3$, and co-cultured ADMSCs treated with TGF-${\beta}3$. Methods : ADMSCs were sub-cultured to homogeneity and used in fluorocytometry assays for CD11, CD45, and CD90/Thy1. ADMSCs were differentiated in spheroid culture towards the chondrogenic lineage by the presence of TGF-${\beta}3$, dexamethasone, and ascorbate. We also co-cultured pure ADMSCs and nucleus pulposus cells in 24-well plates, and performed immunohistochemical staining, western blotting, and RT-PCR for quantitative analysis of gene expression. Results : Results of fluorocytometry were positive for CD90/Thy1 and negative for CD11 and CD45. TGF-${\beta}3$-mediated induction of ADMSCs led to the expression of the differentiation markers of intervertebral disc-like cells, such as aggrecan, collagen II, and sox-9. Co-cultured ADMSCs treated with TGF-${\beta}3$ showed higher expression of differentiation markers and greater extracellular matrix production compared with ADMSCs treated with TGF-${\beta}3$ alone. Conclusion : ADMSC treated with TGF-${\beta}3$ may be an attractive source for regeneration therapy in degenerative IVD. These findings may also help elucidate the pathologic mechanism of MSC therapy in the degeneration of IVD in vivo.
#Adipose tissue #Mesenchymal stem cells #Intervertebral disc #Cell and tissue engineering

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참고문헌 (11)

참고문헌 신청
Hunter CJ / 2003 / The notochordal cell in the nucleus pulposus : a review in the context of tissue engineering / Tissue Eng 9:667~677 google schola Izadpanah R / 2006 / Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue / J Cell Biochem 99:1285~1297 google schola Kern S / 2006 / Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue / Stem Cells 24:1294~1301 google schola Le Visage C / 2006 / Interaction of human mesenchymal stem cells with disc cells : changes in extracellular matrix biosynthesis / Spine 31:2036~2042 google schola Leung VY / 2006 / Regeneration of intervertebral disc by mesenchymal stem cells : potentials, limitations, and future direction / Eur Spine J 15(Suppl 3):S406~S413 google schola

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