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논문 기본 정보

자료유형
학술저널
저자정보
Rahman, Md. Atikur (Division of Applied Life Sciences [BK21Plus], IALS, PMBBRC, Gyeongsang National University) Kim, Yong-Goo (Division of Applied Life Sciences [BK21Plus], IALS, PMBBRC, Gyeongsang National University) Lee, Byung-Hyun (Division of Applied Life Sciences [BK21Plus], IALS, PMBBRC, Gyeongsang National University)
저널정보
한국초지조사료학회 한국초지조사료학회지 한국초지조사료학회지 제34권 제4호
발행연도
2014.1
수록면
262 - 268 (7page)

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In order to reveal the aluminum (Al) stress tolerance mechanisms in alfalfa plant at low pH soil, a proteomic approach has been conducted. Alfalfa plants were exposed to Al stress for 5 days. The plant growth and total chlorophyll content are greatly affected by Al stress. The malondialdehyde (MDA) and $H_2O_2$ contents were increased in a low amount but free proline and soluble sugar contents, and the DPPH-radical scavenging activity were highly increased. These results indicate that antioxidant activity (DPPH activity) and osmoprotectants (proline and sugar) may involve in ROS ($H_2O_2$) homeostasis under Al stress. In proteomic analysis, over 500 protein spots were detected by 2-dimentional gel electrophoresis analysis. Total 17 Al stress-induced proteins were identified, of which 8 protein spots were up-regulated and 9 were down-regulated. The differential expression patterns of protein spots were selected and analyzed by the peptide mass fingerprinting (PMF) using MALDI-TOF MS analysis. Three protein spots corresponding to Rubisco were significantly down-regulated whereas peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress. The different regulation patterns of identified proteins were involved in energy metabolism and antioxidant / ROS detoxification during Al stress in alfalfa. Taken together, these results provide new insight to understand the molecular mechanisms of alfalfa plant in terms of Al stress tolerance.

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