지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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A degenerated strain of Pleurotus eryngii KNR2312 was isolated from a commercial farm. Random amplified polymorphic DNA analysis performed on the genomic DNA of the normal and degenerated strains of this species revealed differences in the DNA banding pattern. A unique DNA fragment (1.7 kbp), which appeared only in the degenerated strain, was isolated and sequenced. Comparing this sequence with the KNR2312 genomic sequence showed that the sequence of the degenerated strain comprised three DNA regions that originated from nine distinct scaffolds of the genomic sequence, suggesting that chromosome-level changes had occurred in the degenerated strain. Using the unique sequence, three sets of PCR primers were designed that targeted the full length, the 5' half, and the 3' half of the DNA. The primer sets P2-1 and P2-2 yielded 1.76 and 0.97 kbp PCR products, respectively, only in the case of the degenerated strain, whereas P2-3 generated a 0.8 kbp product in both the normal and the degenerated strains because its target region was intact in the normal strain as well. In the case of the P2-1 and P2-2 sets, the priming regions of the forward and reverse primers were located at distinct genomic scaffolds in the normal strain. These two primer sets specifically detected the degenerate strain of KNR2312 isolated from various mushrooms including 10 different strains of P. eryngii, four strains of P. ostreatus, and 11 other wild mushrooms.
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참고문헌 (14)
참고문헌 신청
Chang-Yun Lee / 2009 / Determination of Mineral Components in the Cultivation Substrates of Edible Mushrooms and Their Uptake into Fruiting Bodies / Mycobiology 37(2):109~113
Yunjung Lim / 2008 / Isolation of Bacteria Associated with the King Oyster Mushroom, Pleurotus eryngii / Mycobiology 36(1):13~18
Heath MC / 1995 / Hyphal morphology associated with strain instability in the commercial mushroom, Agaricus bisporus / Mycologia 87:442~450
Li A / 1994 / Inheritance of strain instability (sectoring) in the commercial button mushroom, Agaricus bisporus / Appl Environ Microbiol 60:2384~2388
Horgen PA / 1996 / Chromosomal abnormalities associated with strain degeneration in the cultivated mushroom, Agaricus bisporus / Fungal Genet Biol 20:229~241
Yunjung Lim / 2008 / Isolation of Bacteria Associated with the King Oyster Mushroom, Pleurotus eryngii / Mycobiology 36(1):13~18
Heath MC / 1995 / Hyphal morphology associated with strain instability in the commercial mushroom, Agaricus bisporus / Mycologia 87:442~450
Li A / 1994 / Inheritance of strain instability (sectoring) in the commercial button mushroom, Agaricus bisporus / Appl Environ Microbiol 60:2384~2388
Horgen PA / 1996 / Chromosomal abnormalities associated with strain degeneration in the cultivated mushroom, Agaricus bisporus / Fungal Genet Biol 20:229~241
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