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논문 기본 정보

자료유형
학술저널
저자정보
Deguchi, Seitaro (Graduate School of Environmental Studies, Nagoya University) Matsuda, Yosuke (Graduate School of Bioresources, Mie University) Takenaka, Chisato (Graduate School of Bioagricultural Sciences, Nagoya University) Sugiura, Yuki (Graduate School of Bioagricultural Sciences, Nagoya University) Ozawa, Hajime (Fukushima Prefectural Forestry Research Centre) Ogata, Yoshimune (Radioisotope Research Center, Medical Branch, Nagoya University)
저널정보
한국균학회 Mycobiology Mycobiology 제45권 제1호
발행연도
2017.1
수록면
15 - 19 (5page)

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This study proposed a rapid method to quantify the colonization rate of arbuscular mycorrhizal fungi (AMF) in plant roots. The method involved the use of an image analysis software (WinRHIZO Pro). The colonization rate is defined as the ratio of the fungal body to the plant root area in a micrograph. Three seedlings of Chengiopanax sciadophylloides, a woody species that accumulates radiocesium, were collected from a secondary forest in the Yamakiya district of Kawamata, Fukushima Prefecture during May-September 2014. The colonization of AMF structures was examined under a light microscope, and the percentage of colonization was determined using the WinRHIZO method. The superiority of the new method was verified by comparing with a modified grid-line intersect method. The colonization of AMF was confirmed in all the seedlings, and a significant coefficient of determination ($R^2=0.94$) was found with both the methods. The results suggested that the WinRHIZO method is reliable for estimating the colonization of AMF in C. sciadophylloides.

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