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논문 기본 정보

자료유형
학술저널
저자정보
Cha, Hye-Geun (Department of Biotechnology, Korea National University of Transportation) Kim, Yi-Ok (Department of Biotechnology, Korea National University of Transportation) Choi, Woon Yong (Department of Medical Biomaterials Engineering, Kangwon National University) Kang, Do-Hyung (Korea Institute of Ocean Science and Technology) Lee, Hyeon-Yong (Department of Food Science and Engineering, Seowon University) Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation)
저널정보
한국균학회 Mycobiology Mycobiology 제42권 제3호
발행연도
2014.1
수록면
249 - 255 (7page)

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We evaluated a more practical and cost-effective immobilization carriers for ethanol production using the yeast Saccharomyces cerevisiae. Three candidate materials-rice hull, rice straw, and sawdust-were tested for their cell-adsorption capacity and operational durability. Derivatizations of rice hull, rice straw, and sawdust with the optimal concentration of 0.5 M of 2-(diethylamino)ethyl chloride hydrochloride (DEAE HCl) resulted in > 95% adsorption of the initial yeast cells at 2 hr for DEAE-rice hull and DEAE-sawdust and in only approximately 80% adsorption for DEAE-rice straw. In addition, DEAE-sawdust was found to be a more practical carrier for immobilizing yeast cells in terms of operational durability in shaking flask cultures with two different speeds of 60 and 150 rpm. Furthermore, the biosorption isotherms of DEAE-rice hull, -rice straw, and -sawdust for yeast cells revealed that the $Q_{max}$ of DEAE-sawdust (82.6 mg/g) was greater than that of DEAE-rice hull and DEAE-rice straw. During the 404-hr of continuous column reactor operation using yeast cells immobilized on DEAE-sawdust, no serious detachment of the yeast cells from the DEAE-sawdust was recorded. Ethanol yield of approximately 3.04 g/L was produced steadily, and glucose was completely converted to ethanol at a yield of 0.375 g-ethanol/g-glucose (73.4% of the theoretical value). Thus, sawdust is a promising practical immobilization carrier for ethanol production, with significance in the production of bioethanol as a biofuel.

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