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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Entomopathogenic fungi are promising pest-control agents but their industrial applicability is limited by their thermosusceptibility. With an aim to increase the thermotolerance of Isaria fumosorosea SFP-198, moisture absorbents were added to dried conidial powder, and the relationship between its water potential and thermotolerance was investigated. Mycotized rice grains were dried at $10^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$, and $40^{\circ}C$ and the drying effect of each temperature for 24, 48, 96, and 140 hr was determined. Drying for 48 hr at $10^{\circ}C$ and $20^{\circ}C$ reduced the moisture content to < 5% without any significant loss of conidial thermotolerance, but drying at $30^{\circ}C$ and $40^{\circ}C$ reduced both moisture content and conidial thermotolerance. To maintain thermotolerance during storage, moisture absorbents, such as calcium chloride, silica gel, magnesium sulfate, white carbon, and sodium sulfate were individually added to previously dried-conidial powder at 10% (w/w). These mixtures was then stored at room temperature for 30 days and subjected to $50^{\circ}C$ for 2 hr. The white carbon mixture had the highest conidial thermotolerance, followed by silica gel, magnesium sulfate, and then the other absorbents. A significant correlation between the water potential and conidial thermotolerance was observed in all conidia-absorbent mixtures tested in this study (r = -0.945). Conidial thermotolerance in wet conditions was evaluated by adding moisturized white carbon (0~20% $H_2O$) to conidia to mimic wet conditions. Notably, the conidia still maintained their thermotolerance under these conditions. Thus, it is evident that conidial thermotolerance can be maintained by drying mycotized rice grains at low temperatures and adding a moisture absorbent, such as white carbon.
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참고문헌 (29)
참고문헌 신청
United States Environmental Protection Agency / Biopesticide Active Ingredient Fact Sheets
Yatin BT / 2006 / The new biopesticide market
Burges HD / 1998 / Formulation of microbial biopesticides: beneficial microorganisms, nematodes and seed treatment / Kluwer Academic :131~186
Devi KU / 2005 / Effect of high temperature and water stress on in vitro germination and growth in isolate of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin / J Invertebr Pathol 88:181~189
Kim JS / 2010 / Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture / J Ind Microbiol Biotechnol 37:419~423
Yatin BT / 2006 / The new biopesticide market
Burges HD / 1998 / Formulation of microbial biopesticides: beneficial microorganisms, nematodes and seed treatment / Kluwer Academic :131~186
Devi KU / 2005 / Effect of high temperature and water stress on in vitro germination and growth in isolate of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin / J Invertebr Pathol 88:181~189
Kim JS / 2010 / Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture / J Ind Microbiol Biotechnol 37:419~423
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