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논문 기본 정보

자료유형
학술저널
저자정보
Zhang, Xiaoping (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Zhang, Bo (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Miao, Renyun (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Zhou, Jie (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Ye, Lei (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Jia, Dinghong (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Peng, Weihong (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Yan, Lijuan (Aquatic Geomicrobiology, Institute of Biodiversity, Friedrich Schiller University) Zhang, Xiaoping (Department of Microbiology, College of Resources, Sichuan Agricultural University) Tan, Wei (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences) Li, Xiaolin (Soil and Fertilizer Institute, Sichuan Academy of Agriculture Sciences)
저널정보
한국균학회 Mycobiology Mycobiology 제46권 제3호
발행연도
2018.1
수록면
224 - 235 (12page)

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Temperature is an important environmental factor that can greatly influence the cultivation of Auricularia cornea. In this study, lignin peroxidase, laccase, manganese peroxidase, and cellulose in A. cornea fruiting bodies were tested under five different temperatures ($20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, $35^{\circ}C$, and $40^{\circ}C$) in three different culture periods (10 days, 20 days and 30 days). In addition, the V4 region of bacterial 16S rRNA genes in the substrate of A. cornea cultivated for 30 days at different temperatures were sequenced using next-generation sequencing technology to explore the structure and diversity of bacterial communities in the substrate. Temperature and culture days had a significant effect on the activities of the four enzymes, and changes in activity were not synchronized with changes in temperature and culture days. Overall, we obtained 487,694 sequences from 15 samples and assigned them to 16 bacterial phyla. Bacterial community composition and structure in the substrate changed when the temperature was above $35^{\circ}C$. The relative abundances of some bacteria were significantly affected by temperature. A total of 35 genera at five temperatures in the substrate were correlated, and 41 functional pathways were predicted in the study. Bacterial genes associated with the membrane transport pathway had the highest average abundance (16.16%), and this increased at $35^{\circ}C$ and $40^{\circ}C$. Generally, different temperatures had impacts on the physiological activity of A. cornea and the bacterial community in the substrate; therefore, the data presented herein should facilitate cultivation of A. cornea.

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