지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial identification. In this study, we applied SCAR markers for the rapid and accurate detection of Phytophthora katsurae, the casual agent of chestnut ink disease in Korea. In this study, we developed seven SCAR markers specific to P. katsurae using random amplified polymorphic DNA (RAPD), and assessed the potential of the SCAR markers to serve as tools for identifying P. katsurae. Seven primer pairs (SOPC 1F/SOPC 1R, SOPC 1-1F/SOPC 1-1R, SOPC 3F/SOPC 3R, SOPC 4F/SOPC 4R, SOPC 4F/SOPC 4-1R, SOPD 9F/SOPD 9R, and SOPD 10F/SOPD 10R) from a sequence derived from RAPD fragments were designed for the analysis of the SCAR markers. To evaluate the specificity and sensitivity of the SCAR markers, the genomic DNA of P. katsurae was serially diluted 10-fold to final concentrations from 1 mg/mL to 1 pg/mL. The limit of detection using the SCAR markers ranged from $100{\mu}g/mL$ to 100 ng/mL. To identify the limit for detecting P. katsurae zoospores, each suspension of zoospores was serially diluted 10-fold to final concentrations from $10{\times}10^5$ to $10{\times}10^1$ zoospores/mL, and then extracted. The limit of detection by SCAR markers was approximately $10{\times}10^1$ zoospores/mL. PCR detection with SCAR markers was specific for P. katsurae, and did not produce any P. katsurae-specific PCR amplicons from 16 other Phytophthora species used as controls. This study shows that SCAR markers are a useful tool for the rapid and effective detection of P. katsurae.
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등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Oh ES / 2007 / Chestnut ink disease caused by Phytophthora katsurae / J For Sci 23:65~71
Boutard A. / 2001 / The western chestnut: more information on chestnuts and ink disease. Vol. 3 / The Western Chestnut Growers Assn., Inc :1
이종규 / 2009 / Isolation, Identification and Characterization of Phytophthora katsurae, Causing Chestnut Ink Disease in Korea / The Plant Pathology Journal 25(2):121~127
Robideau GP / 2011 / DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer / Mol Ecol Resour 11:1002~1011
Schubert R / 1999 / Detection and quantification of Phytophthora species which are associated with root-rot disease in European deciduous forests by species-specific polymerase chain reaction / Eur J Plant Pathol 29:169~188
Boutard A. / 2001 / The western chestnut: more information on chestnuts and ink disease. Vol. 3 / The Western Chestnut Growers Assn., Inc :1
이종규 / 2009 / Isolation, Identification and Characterization of Phytophthora katsurae, Causing Chestnut Ink Disease in Korea / The Plant Pathology Journal 25(2):121~127
Robideau GP / 2011 / DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer / Mol Ecol Resour 11:1002~1011
Schubert R / 1999 / Detection and quantification of Phytophthora species which are associated with root-rot disease in European deciduous forests by species-specific polymerase chain reaction / Eur J Plant Pathol 29:169~188
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