지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 710 - 717 (8page)
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초록· 키워드
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Anti-inflammatory effects of methanol extract and solvent fractions of cowpea (Vigna sinensis K.; VS) seeds and the isolated compounds were evaluated. Ethyl acetate and n-butanol fractions of VS seeds were found to strongly inhibit nitric oxide (NO) production, and inducible nitric oxide synthase (iNOS) mRNA and protein expressions in lipopolysaccharides (LPS)-stimulated RAW264.7 macrophage cells. Compounds inhibiting NO production in RAW264.7 cells were isolated and identified via successive partitioning and spectroscopic characterization. The active compounds included oleanolic acid, linolenic acid (LnA), linoleic acid (LA), 7-ketositosterol, stigmasterol-glucose (glc), and soyasaponin 1. Among the isolated compounds, LnA and LA were found to inhibit NO production significantly. Contents of LnA and LA in VS seeds were 2.034 and 1.162 mg/g on dry weight basis, respectively. Reverse transcriptase-polymerase chain reaction (RTPCR) and Western blot analysis show inhibitory effects of these compounds. LnA and LA repressed mRNA and protein levels of iNOS in LPS-stimulated cells. LA suppressed the production of pro-inflammatory cytokines such as interleukin (IL)-1${\beta}$, IL-6, and tumor necrosis factor (TNF)-${\alpha}$ in LPS-induced macrophage cells. Thus, VS was found to exhibit strong anti-inflammatory activity, which may be partly attributed to the polyunsaturated fatty acids such as LnA and LA.
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