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논문 기본 정보

자료유형
학술저널
저자정보
Jeong, Jun-Mo (Department of Oriental Medicine Materials, Dongshin University) Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University) Kim, Sun-Min (Department of Oriental Medicine Materials, Dongshin University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제1호
발행연도
2014.1
수록면
89 - 93 (5page)

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We aim to develop a simple method for simultaneous and quantitative determination of benzoic acid, caffeic acid and chlorogenic acid in seeds of Eriobotrya japonica. In addition, antibacterial effect of these three phenolic acids was examined. A basic method is performed on the high performance liquid chromatography system coupled to an UV-detector (230 nm) and reverse phase C-18 column ($4.6{\times}150mm$, $5{\mu}m$). Each phenolic acid was confirmed via liquid chromatography-mass spectrometry (MS)/MS system under the multiple-reaction monitoring with negative-ion electrospray ionization (ESI(-)) mode. It is demonstrated that the method was could be applied to samples for an analytical study of the phenolic acids. On the other hand, three phenolic acids in seeds of E. japonica exhibited antibacterial effect against several pathogenic bacteria. Of these, benzoic acid was found to have stronger antibacterial effect.

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