메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
Nguyen, Dang-Minh-Chanh (Western Highlands Agro-Forestry Scientific and Technical Institute) Seo, Dong-Jun (Division of Applied Bioscience and Biotechnology, Environment-Friendly Agriculture Research Center [EFARC], Institute of Agricultural Science and Technology, Chonnam National University) Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Environment-Friendly Agriculture Research Center [EFARC], Institute of Agricultural Science and Technology, Chonnam National University) Lee, Bok-Rye (Department of Animal Sciences, Institute of Agricultural Science and Technology, Chonnam National University) Jung, Woo-Jin (Division of Applied Bioscience and Biotechnology, Environment-Friendly Agriculture Research Center [EFARC], Institute of Agricultural Science and Technology, Chonnam National University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제54권 제4호
발행연도
2011.1
수록면
507 - 514 (8page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
Cucumber plants were infected with the root-knot nematode Meloidogyne incognita and were then treated with Cinnamomum cassia crude extracts (CCE). The activities of antioxidative enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), and ascorbate peroxidase (APX, EC 1.11.1.11, class I peroxidase) were detected in the cucumber leaves. On day 28 after treatment of C. cassia (0, 1, 5, and 10 mg/mL), the relative galling formation decreased significantly in a dose-dependent manner. The C. cassia crude extracts also effectively inhibited the growth of M. incognita in soil and roots. The number of second-stage juveniles was significantly decreased as the concentration of the C. cassia crude extracts increased. Twenty-eight days after treatment with CCE, the SOD activities of plant leaves treated with 5 and 10 mg/mL CCE were greater than that of leaves treated with nematode (Ne) only. In addition, the CAT and ascorbate peroxidase (APX) activities increased as the concentration of CCE increased. The relative activities of the antioxidative enzymes decreased as the galling formation in the roots increased in response to M. incognita infection. Polyacrylamide gel electrophoresis (PAGE) with activity staining revealed active bands of SOD corresponding to at least five isozymes (93, 79, 71, 66, and 41 kDa), including three Fe-SOD bands (71, 66, and 41 kDa) and two Mn-SOD bands (93 and 79 kDa). Analysis of the cucumber leaves revealed one active band corresponding to the CAT isozyme (>97 kDa) on 10% native gels. At least four bands corresponding to APX were observed in 10% polyacrylamide, including one major band (>97 kDa) and three minor bands (79, 71, and 66 kDa). Compared with the leaves of cucumber plants that received other treatments, SOD (41 kDa) and APX (70 kDa) isozymes in the leaves of plants treated with Ne only showed intensified bands in PAGE gels.

목차

등록된 정보가 없습니다.

참고문헌 (0)

참고문헌 신청

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0