지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 325 - 331 (7page)
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초록· 키워드
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Paenibacillus sp. DZ3, newly isolated from Konjack field, produced ${\beta}$-mannanase (900 U/mL) when grown on glucomannan as a carbon source. ${\beta}$-Mannanase was purified 34-fold to homogeneity resulting in final recovery of 15% and specificity of 169 U/mg protein. The molecular mass was approximately 39 kDa as estimated by sodiumdodecylsulfate-polyacrylamide gel electrophoresis. Active band was observed as clear colourless area on zymogram. The optimal temperature and pH for mannanase activity was $60^{\circ}C$ and pH 5.0, respectively. The activity was stable up to $60^{\circ}C$ at pH 5.0 and remained stable from pH 5.0-7.0. Mannanase was highly specific towards glucomannan and galactomannan, whereas exhibited low activity towards mushroom powder. The Michaelis constant ($K_m$) and maximum velocity ($V_{max}$) for glucomannan substrate were 1.05 mg/mL and 714 U/mg, respectively. These results indicate the enzyme is attractive for industrial applications.
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