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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제3호
- 발행연도
- 2014.1
- 수록면
- 197 - 203 (7page)
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초록· 키워드
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Various parameters such as solvent selection, concentration, solid/liquid ratio, soaking time, temperature, stationary, shaking conditions, and repeated extractions were investigated in order to determine the optimum extraction conditions of ${\beta}$-glucosidase from bagasse fermented by mixed culture of Aspergillus niger NRC 7A and Aspergillus oryzae NRRL 447. Among various solvents tested, non ionic detergents gave the best results than the inorganic or organic salt solutions and distilled water. The optimum conditions for extraction of ${\beta}$-glucosidase were 30 min soaking time at $40^{\circ}C$ under shaking condition at 150 rpm, with solid/liquid ratio 1:15 (w/v), which yielded $2882.74{\pm}95.52U/g$ fermented culture (g fc) of enzyme activity. With repeated washes under the above optimum conditions, the results showed that enzyme extracted in the $1^{st}$ and $2^{nd}$ washes represents about 90% of the total activity.
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참고문헌 (35)
참고문헌 신청
Ahmed A / 2008 / Optimization of production and extraction parameters of Bacillus megaterium levansucrase using solid state fermentation / J Appl Sci Res 4(10):1199~1204
Annunziato ME / 1370 / Production of α-galactosidase from Aspergillus oryzae grown in solid state culture / J Food Sci 51:1370
Cai YJ / 1999 / Production and distribution of endo-β-glucanase, cellobiohydrolase and β-glucosidase components of the cellulolytic system of Volvariella volvacea, the edible straw mushroom / Appl Environ Microbiol 65(2):553~559
Camassola M / 2007 / Production of cellulases and hemicellulases by Penicillium echinulatum grown on pretreated sugar cane bagasse and wheat bran in solid-state fermentation / J Appl Microbiol 103:2196~2204
Castilho LR / 1999 / Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger / Process Biochem 34:181~186
Annunziato ME / 1370 / Production of α-galactosidase from Aspergillus oryzae grown in solid state culture / J Food Sci 51:1370
Cai YJ / 1999 / Production and distribution of endo-β-glucanase, cellobiohydrolase and β-glucosidase components of the cellulolytic system of Volvariella volvacea, the edible straw mushroom / Appl Environ Microbiol 65(2):553~559
Camassola M / 2007 / Production of cellulases and hemicellulases by Penicillium echinulatum grown on pretreated sugar cane bagasse and wheat bran in solid-state fermentation / J Appl Microbiol 103:2196~2204
Castilho LR / 1999 / Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger / Process Biochem 34:181~186
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