Mechanism for Antioxidant Activity of Nardostachys chinensis root Extract

Heo, Jee-In; Kim, Jeong-Hyeon; Lee, Jeong-Min; Kim, Sung Chan; Park, Jae-Bong; Kim, Jaebong; Lee, Jae-Yong

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자료유형: 학술저널

저자정보: Heo, Jee-In (Department of Biochemistry, Hallym University Medical School) Kim, Jeong-Hyeon (Department of Biochemistry, Hallym University Medical School) Lee, Jeong-Min (Department of Biochemistry, Hallym University Medical School) Kim, Sung Chan (Department of Biochemistry, Hallym University Medical School) Park, Jae-Bong (Department of Biochemistry, Hallym University Medical School) Kim, Jaebong (Department of Biochemistry, Hallym University Medical School) Lee, Jae-Yong (Department of Biochemistry, Hallym University Medical School)

저널정보: 한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제1호

발행연도: 2014.1

수록면: 17 - 22 (6page)

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Nardostachys chinensis (N. chinensis) has been used in traditional medicine as a sedative and analgesic. It has been reported that N. chinensis extract has an antioxidant activity. However, the mechanism has not been elucidated. In this study, we showed that FOXO3a was activated by N. chinensis extract. FOXO3a is a transcriptional factor that involved in cell cycle arrest, DNA repair, apoptosis, and detoxification of reactive oxygen spices (ROS). Protein level of FOXO3a was increased by N. chinensis extract whereas phospho-FOXO3a (Thr 32) was not changed. Promoter activities of target genes of FOXO3a such as MnSOD, p27, and GADD45 were increased by N. chinensis extract. Among target genes, protein level of MnSOD was increased by N. chinensis extract, and this leads to removal of ROS level in human embryonic fibroblast (HEF) cells. These results suggested that N. chinensis extract has an antioxidant activity by upregulation of MnSOD through FOXO3a activation.

#antioxidant #FOXO3a #MnSOD #Nardostachys chinensis #reactive oxygen species

참고문헌 (29)

참고문헌 신청

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