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학술저널
저자정보
Lee, Min-Kyoung (Department of Agricultural Chemistry, Chungbuk National University) Chauhan, Puneet Singh (Department of Agricultural Chemistry, Chungbuk National University) Yim, Woo-Jong (Department of Agricultural Chemistry, Chungbuk National University) Lee, Gyeong-Ja (Chungbuk Agricultural Research and Extension Services) Kim, Young-Sang (Chungbuk Agricultural Research and Extension Services) Park, Kee-Woong (Bio-Evaluation Center, KRIBB) Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제54권 제2호
발행연도
2011.1
수록면
120 - 125 (6page)

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In order to exploit Methylobacterium oryzae CBMB20 as of plant growth promoting agent, different inoculation methods have been evaluated. The present study aimed to evaluate soil, foliar, and soil+foliar inoculations of M. oryzae CBMB20 to improve the growth, fruit yield, and nutrient uptake of red pepper (Capsicum annuum L.) under greenhouse conditions. The population range of green fluorescent protein (gfp)-tagged M. oryzae CBMB20 using the three inoculation methods was 2.5-2.9 ${\log}_{10}$ cfu/g in the rhizosphere and 4.5-6.0 ${\log}_{10}$ cfu/g in the phyllosphere of red pepper plants. Confocal laser scanning microscopy results confirmed the colonization of M. oryzae CBMB20 endophytically on leaf surface. Plant height, fruit dry weight, and total biomass were significantly higher ($p{\leq}0.05$) in all M. oryzae CBMB20 inoculation methods as compared to non-inoculated control. Furthermore, uptake of mineral nutrients such as N, P, K, Ca, and Mg in red pepper plants in all M. oryzae CBMB20 inoculation methods was higher than in non-inoculated control. Comparative results of inoculation methods clearly demonstrated that soil+foliar inoculation of M. oryzae CBMB20 lead to the highest biomass accumulation and nutrient uptake which may be due to its efficient colonization in the red pepper rhizosphere and phyllosphere.

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