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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제2호
- 발행연도
- 2014.1
- 수록면
- 137 - 140 (4page)
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초록· 키워드
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A gene encoding thermostable pectinase (TmPec) was isolated from hyperthermophilic microorganism, Thermotoga maritima. The open reading frame (ORF) of TmPec gene is 1,104 bp long and encodes 367 amino acid residues with a molecular weight of 40,605 Da. To analyze the enzymatic activity and biochemical properties, the ORF of TmPec gene excluding putative signal sequence of 27 amino acids was introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. Protein concentration of purified recombinant TmPec was 1.1 mg/mL with specific activity of 56 U/mg protein on pectin. The recombinant TmPec showed the highest activity at around $85-95^{\circ}C$, and at around pH 6.5. It was stable at temperature below $85^{\circ}C$. In the presence of $Ca^{2+}$, the activity of recombinant TmPec was increased to 146.3% of normal level. In contrast, $Ba^{2+}$ and Mn2+ showed strong inhibition to the recombinant TmPec.
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참고문헌 (23)
참고문헌 신청
Alkorta I / 1998 / Industrial applications of pectic enzymes: a review / Process Biochem 33:21~28
Beg QK / 2000 / Production and characterization of thermostable xylanase and pectinase from a Streptomyces sp. QG-11-3 / J Ind Microbiol Biotechnol 24:396~402
Blanco P / 1999 / Production of pectic enzymes in yeasts / FEMS Microbiol Lett 175:1~9
Bonch-Osmolovskaya EA / 1998 / Characteristics of Desulfurococcus amyloliticus sp. nov., a new extremely thermophlilic archaebacterium isolated from thermal springs of Kamchatka and Kunashir lsland / Mikrobiologiya 57:94~101
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Beg QK / 2000 / Production and characterization of thermostable xylanase and pectinase from a Streptomyces sp. QG-11-3 / J Ind Microbiol Biotechnol 24:396~402
Blanco P / 1999 / Production of pectic enzymes in yeasts / FEMS Microbiol Lett 175:1~9
Bonch-Osmolovskaya EA / 1998 / Characteristics of Desulfurococcus amyloliticus sp. nov., a new extremely thermophlilic archaebacterium isolated from thermal springs of Kamchatka and Kunashir lsland / Mikrobiologiya 57:94~101
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
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