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자료유형
학술저널
저자정보
Zhou, Qing-Fen (College of Biology and Sciences, Sichuan Agricultural University) Jia, Xue-Jing (College of Biology and Sciences, Sichuan Agricultural University) Li, Qian-Qian (College of Biology and Sciences, Sichuan Agricultural University) Yang, Rui-Wu (College of Biology and Sciences, Sichuan Agricultural University) Zhang, Li (College of Biology and Sciences, Sichuan Agricultural University) Zhou, Yong-Hong (Laboratory of Crop Genetic Resources and Improvement Ministry of Education, Sichuan Agricultural University) Ding, Chun-Bang (College of Biology and Sciences, Sichuan Agricultural University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제2호
발행연도
2014.1
수록면
123 - 129 (7page)

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The antioxidant and antimicrobial activities of Camellia oleifera seed oil were studied. Four kinds of seed oil samples were prepared, crude oil and refined oil, extracted by cold pressing method (CPC, CPR), and organic solvent extraction (OSC, OSR). Antioxidant activity analysis was measured in 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, ferric reducing Ability of Plasma, and 2,2-diphenyl-1-picrylhydrazyl assays. Besides, the percentage of inhibition of red blood cells hemolysis induced by 2,2'-azobis(2-amidnopropane) dihydrochlorid, the lag time of LDL conjugated dienes formation in vitro, and the inhibitors of loss in tryptophan fluorescence were all used to estimate the antioxidant activity of the samples. The total phenolic contents (TPC) were detemined by Folin-Ciocalteu method. The TPC of the C. oleifera seed oils can be arranged in descending order: CPC ($1.9172{\mu}g/mL$) > OSC ($1.5218{\mu}g/mL$) > CPR ($1.0611{\mu}g/mL$) > OSR ($0.6782{\mu}g/mL$). And the oils were investigated for activity against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The results showed the antioxidant activity of crude oil by cold pressing method was stronger than others, and all oils did inhibit activity of the top three bacteria expert A. niger. The further significance of the study contributes to measure the antioxidant and antimicrobial activity of the potential health benefits by the different methods of preparation and the oil of C. oleifera seeds acting as free radical scavenger, pharmaceuticals and preservatives may offer some information in medicine and cosmetic not just in food field.

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