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논문 기본 정보

자료유형
학술저널
저자정보
Guven, Mustafa (Department of Neurosurgery, Canakkale Onsekiz Mart University, Faculty of Medicine) Akman, Tarik (Department of Neurosurgery, Canakkale Onsekiz Mart University, Faculty of Medicine) Yener, Ali Umit (Department of Cardiovascular Surgery, Canakkale Onsekiz Mart University, Faculty of Medicine) Sehitoglu, Muserref Hilal (Department of Medical Biochemistry, Canakkale Onsekiz Mart University, Faculty of Medicine) Yuksel, Yasemin (Department of Histology and Embryology, Afyon Kocatepe University, Faculty of Medicine) Cosar, Murat (Department of Neurosurgery, Canakkale Onsekiz Mart University, Faculty of Medicine)
저널정보
대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제57권 제5호
발행연도
2015.1
수록면
335 - 341 (7page)

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Objective : The main causes of spinal cord ischemia are a variety of vascular pathologies causing acute arterial occlusions. We investigated neuro-protective effects of kefir on spinal cord ischemia injury in rats. Methods : Rats were divided into three groups : 1) sham operated control rats; 2) spinal cord ischemia group fed on a standard diet without kefir pretreatment; and 3) spinal cord ischemia group fed on a standard diet plus kefir. Spinal cord ischemia was performed by the infrarenal aorta cross-clamping model. The spinal cord was removed after the procedure. The biochemical and histopathological changes were observed within the samples. Functional assessment was performed for neurological deficit scores. Results : The kefir group was compared with the ischemia group, a significant decrease in malondialdehyde levels was observed (p<0.05). Catalase and superoxide dismutase levels of the kefir group were significantly higher than ischemia group (p<0.05). In histopathological samples, the kefir group is compared with ischemia group, there was a significant decrease in numbers of dead and degenerated neurons (p<0.05). In immunohistochemical staining, hipoxia-inducible factor-$1{\alpha}$ and caspase 3 immunopositive neurons were significantly decreased in kefir group compared with ischemia group (p<0.05). The neurological deficit scores of kefir group were significantly higher than ischemia group at 24 h (p<0.05). Conclusion : Our study revealed that kefir pretreatment in spinal cord ischemia/reperfusion reduced oxidative stress and neuronal degeneration as a neuroprotective agent. Ultrastructural studies are required in order for kefir to be developed as a promising therapeutic agent to be utilized for human spinal cord ischemia in the future.

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