지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 281 - 287 (7page)
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초록· 키워드
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UNC93B1 is involved in the delivery of nucleotide-sensing Toll-like receptors (TLR) including TLR9 to endolysosomes. However, possible functions of UNC93A, another member of the UNC-93 superfamily, have not been studied in the context of TLR signaling. Here, we injected naked CpG-ODNs or liposome-encapsulated CpG-ODN (Lipoplex(O)) into the BALB/c mouse peritoneal cavity and investigated expression of mouse UNC93B1 and UNC93A genes in the peritoneal cells. UNC93A mRNA expression was increased by Lipoplex(O) in a time-dependent manner, whereas the expression level of UNC93B1 was not changed. To evaluate whether the expression of UNC93A involves TLR9, TLR9 knock out (TLR-/-) mice were injected with Lipoplex(O) or LipoplexGC(O), and the peritoneal cells were analyzed. The expression of UNC93A was not induced by Lipoplex(O) in TLR9-/- mice. These results suggest that UNC93A is closely associated with TLR9 signaling induced by Lipoplex(O) in the peritoneal cells in vivo. Primary cells including peritoneal cells stimulated with CpG-ODNs, Lipoplex(O), and several cytokines in vitro were prepared. However, UNC93A expression was not induced by any stimulation. To identify cellular localization of UNC93A, human embryonic kidney 293 cells stably expressing UNC93A were established and analyzed by confocal microscopy. The human and mouse UNC93A proteins were detected in the cytoplasm. Further investigation of the UNC93A function related with the CpG-DNA-mediated immune response may provide information to support efficient application of CpG-DNA immunotherapeutics.
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