N-(p-Coumaryol)-Tryptamine Suppresses the Activation of JNK/c-Jun Signaling Pathway in LPS-Challenged RAW264.7 Cells

Vo, Van Anh; Lee, Jae-Won; Park, Jun-Ho; Kwon, Jae-Hyun; Lee, Hee Jae; Kim, Sung-Soo; Kwon, Yong-Soo; Chun, Wanjoo

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자료유형: 학술저널

저자정보: Vo, Van Anh (Department of Pharmacology, College of Medicine, Kangwon National University) Lee, Jae-Won (Department of Pharmacology, College of Medicine, Kangwon National University) Park, Jun-Ho (Department of Pharmacology, College of Medicine, Kangwon National University) Kwon, Jae-Hyun (Department of Pharmacology, College of Medicine, Kangwon National University) Lee, Hee Jae (Department of Pharmacology, College of Medicine, Kangwon National University) Kim, Sung-Soo (Department of Pharmacology, College of Medicine, Kangwon National University) Kwon, Yong-Soo (Department of Pharmacology, College of Pharmacy, Kangwon National University) Chun, Wanjoo (Department of Pharmacology, College of Medicine, Kangwon National University)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제22권 제3호

발행연도: 2014.1

수록면: 200 - 206 (7page)

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N-(p-Coumaryol) tryptamine (CT), a phenolic amide, has been reported to exhibit anti-oxidant and anti-inflammatory activities. However, the underlying mechanism by which CT exerts its pharmacological properties has not been clearly demonstrated. The objective of this study is to elucidate the anti-inflammatory mechanism of CT in lipopolysaccharide (LPS)-challenged RAW264.7 macrophage cells. CT significantly inhibited LPS-induced extracellular secretion of pro-inflammatory mediators such as nitric oxide (NO) and $PGE_2$, and protein expressions of iNOS and COX-2. In addition, CT significantly suppressed LPS-induced secretion of pro-inflammatory cytokines such as TNF-${\alpha}$ and IL-$1{\beta}$. To elucidate the underlying anti-inflammatory mechanism of CT, involvement of MAPK and Akt signaling pathways was examined. CT significantly attenuated LPS-induced activation of JNK/c-Jun, but not ERK and p38, in a concentration-dependent manner. Interestingly, CT appeared to suppress LPS-induced Akt phosphorylation. However, JNK inhibition, but not Akt inhibition, resulted in the suppression of LPS-induced responses, suggesting that JNK/c-Jun signaling pathway significantly contributes to LPS-induced inflammatory responses and that LPS-induced Akt phosphorylation might be a compensatory response to a stress condition. Taken together, the present study clearly demonstrates CT exerts anti-inflammatory activity through the suppression of JNK/c-Jun signaling pathway in LPS-challenged RAW264.7 macrophage cells.

#N- #p-Coumaroyl) tryptamine #RAW 264 #7 cells #Lipopolysaccharide #iNOS #COX-2 #JNK #c-Jun

참고문헌 (38)

참고문헌 신청

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