지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 165 - 170 (6page)
이용수
초록· 키워드
오류제보하기
Cell migration plays a role in many physiological and pathological processes. Reactive oxygen species (ROS) produced in mammalian cells influence intracellular signaling processes which in turn regulate various biological activities. Here, we investigated whether melanoma cell migration could be controlled by ROS production under normoxia condition. Cell migration was measured by wound healing assay after scratching confluent monolayer of B16F10 mouse melanoma cells. Cell migration was enhanced over 12 h after scratching cells. In addition, we found that ROS production was increased by scratching cells. ERK phosphorylation was also increased by scratching cells but it was decreased by the treatment with ROS scavengers, N-acetylcysteine (NAC). Tumor cell migration was inhibited by the treatment with PD98059, ERK inhibitor, NAC or DPI, well-known ROS scavengers. Tumor cell growth as judged by succinate dehydrogenase activity was inhibited by NAC treatment. When mice were intraperitoneally administered with NAC, the intracellular ROS production was reduced in peripheral blood mononuclear cells. In addition, B16F10 tumor growth was significantly inhibited by in vivo treatment with NAC. Collectively, these findings suggest that tumor cell migration and growth could be controlled by ROS production and its downstream signaling pathways, in vitro and in vivo.
목차
등록된 정보가 없습니다.
참고문헌 (33)
참고문헌 신청
Aghajanian, A. / 2009 / Direct activation of RhoA by reactive oxygen species requires a redox-sensitive motif / PLoS One 4:e8045
Borisy, G. G. / 2000 / Actin machinery: pushing the envelope / Curr. Opin. Cell Biol 12:104~112
Cheng, G. C. / 2004 / Oxidative stress and thioredoxin-interacting protein promote intravasation of melanoma cells / Exp. Cell Res 300:297~307
Chetram, M. A. / 2011 / ROS enhances CXCR4-mediated functions through inactivation of PTEN in prostate cancer cells / Biochem. Biophys. Res. Commun 410:195~200
Denizot, F. / 1986 / Rapid colorimetric assay for cell growth and survival. Modifi cations to the tetrazolium dye procedure giving improved sensitivity and reliability / J. Immunol. Methods 89:271~277
Borisy, G. G. / 2000 / Actin machinery: pushing the envelope / Curr. Opin. Cell Biol 12:104~112
Cheng, G. C. / 2004 / Oxidative stress and thioredoxin-interacting protein promote intravasation of melanoma cells / Exp. Cell Res 300:297~307
Chetram, M. A. / 2011 / ROS enhances CXCR4-mediated functions through inactivation of PTEN in prostate cancer cells / Biochem. Biophys. Res. Commun 410:195~200
Denizot, F. / 1986 / Rapid colorimetric assay for cell growth and survival. Modifi cations to the tetrazolium dye procedure giving improved sensitivity and reliability / J. Immunol. Methods 89:271~277
이 논문의 저자 정보
최근 본 자료
댓글(0)
0