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논문 기본 정보

자료유형
학술저널
저자정보
Bai, Yujia (College of Food Science and Pharmacy, College of Forestry and Horticulture, Xinjiang Agricultural University) Huang, Wenshu (College of Food Science and Pharmacy, Xinjiang Agricultural University) Tao, Yongxia (College of Food Science and Pharmacy, Xinjiang Agricultural University) Feng, Zuoshan (College of Food Science and Pharmacy, College of Forestry and Horticulture, Xinjiang Agricultural University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제57권 제1호
발행연도
2014.1
수록면
97 - 103 (7page)

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The precise mechanism by which asafoetida extract influenced the growth of Pleurotus ferulae mycelium was investigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group, the two asafoetida extracts played a significant role in promoting the growth of P. ferulae. Two-dimensional electrophoresis and LC-MS/MS were performed to examine changes in the expression of proteins in the mycelium of P. ferulae. A total of eight differentially expressed proteins were identified, including a heat shock protein, a flavin-containing monooxygenase, and a NADPH: quinone oxidoreductase type IV, as well as hypothetical and unknown proteins. Expression change of these proteins confirmed that asafoetida extracts significantly affected P. ferulae growth, metabolism, and secondary product metabolism. These results provide new insights into biochemical mechanisms underlying the relationship between P. ferulae and asafoetida.

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