지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 129 - 135 (7page)
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초록· 키워드
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Previously, we reported that lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, can increase intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$) via type 1 lysophosphatidic acid (LPA) receptor ($LPA_1$) and CD97, an adhesion G-protein-coupled receptor (GPCR), in MDA-MB-231 breast cancer cells. Furthermore, LPE signaling was suggested as like $LPA_1/CD97-G_{i/o}$ proteins-phospholipase $C-IP_3-Ca^{2+}$ increase in these cells. In the present study, we further investigated actions of LPE not only in the $[Ca^{2+}]_i$ increasing effect but also in cell proliferation and migration in MDA-MB-231 breast cancer cells. We utilized chemically different LPEs and a specific inhibitor of $LPA_1$, AM-095 in comparison with responses in SK-OV3 ovarian cancer cells. It was found that LPE-induced $Ca^{2+}$ response in MDA-MB-231 cells was evoked in a different manner to that in SK-OV3 cells in terms of structural requirements. AM-095 inhibited LPE-induced $Ca^{2+}$ response and cell proliferation in MDA-MB-231 cells, but not in SK-OV3 cells, supporting $LPA_1$ involvement only in MDA-MB-231 cells. LPA had significant effects on cell proliferation and migration in MDA-MB-231 cells, whereas LPE had less or no significant effect. However, LPE modulations of MAPKs (ERK1/2, JNK and p38 MAPK) was not different to those by LPA in the cells. These data support the involvement of LPA1 in LPE-induced $Ca^{2+}$ response and cell proliferation in breast MDA-MB-231 cells but unknown GPCRs (not $LPA_1$) in LPE-induced responses in SK-OV3 cells. Furthermore, although LPE and LPA utilized $LPA_1$, LPA utilized more signaling cascades than LPE, resulting in stronger responses by LPA in proliferation and migration than LPE in MDA-MB-231 cells.
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참고문헌 (22)
참고문헌 신청
Bo Ra Ahn / 2012 / Neuroprotective Effect of Edible Brown Alga Eisenia bicyclis on Amyloid Beta Peptide-induced Toxicity in PC12 Cells / Archives of Pharmacal Research 35(11):1989~1998
Castelino, F. V / 2011 / Amelioration of dermal fibrosis by genetic deletion orpharmacologic antagonism of lysophosphatidic acid receptor 1 ina mouse model of scleroderma / Arthritis Rheum 60:1405~1415
Young-Ja Chang / 2006 / Structure-Activity Relationships of Dimethylsphingosine (DMS) Derivatives and their Effects on Intracellular pH and Ca2+ in the U937 Monocyte Cell Line / Archives of Pharmacal Research 29(8):657~665
Choi, J. W / 2013 / Lysophospholipids and their receptorsin the central nervous system / Biochim. Biophys. Acta 1831:20~32
Inoue, A / 2012 / TGFa shedding assay: an accurate and versatile methodfor detecting GPCR activation / Nature methods 9:1021~1029
Castelino, F. V / 2011 / Amelioration of dermal fibrosis by genetic deletion orpharmacologic antagonism of lysophosphatidic acid receptor 1 ina mouse model of scleroderma / Arthritis Rheum 60:1405~1415
Young-Ja Chang / 2006 / Structure-Activity Relationships of Dimethylsphingosine (DMS) Derivatives and their Effects on Intracellular pH and Ca2+ in the U937 Monocyte Cell Line / Archives of Pharmacal Research 29(8):657~665
Choi, J. W / 2013 / Lysophospholipids and their receptorsin the central nervous system / Biochim. Biophys. Acta 1831:20~32
Inoue, A / 2012 / TGFa shedding assay: an accurate and versatile methodfor detecting GPCR activation / Nature methods 9:1021~1029
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