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자료유형
학술저널
저자정보
Nguyen, Linh Thi Thao (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) Song, Yeon Woo (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) Tran, Thao Anh (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) Kim, Ki-Seok (Department of Medicine, School of Medicine, Jeju National University) Cho, Somi Kim (Subtropical Horticulture Research Institute, Jeju National University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제57권 제4호
발행연도
2014.1
수록면
469 - 472 (4page)

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Anoikis (or cell-detachment-induced apoptosis) is a special form of apoptosis in which cells loose contact with other cells or the extracellular matrix. In the present study, we have found that the breast cancer stem cell line MCF-7-SC, isolated from MCF-7 breast cancer cells, shows characteristics of anoikis resistance. Treatment of supercritical $CO_2$ extract from Citrus hassaku Hort ex Tanaka, locally known as Phalsak, reduced the expression of ATP-binding cassette sub-family G member 2 mRNA and induced apoptosis in MCF-7-SCs, as evidenced by an increase of apoptotic body formation, increased cell population in the sub-G1 phase, increase in the Bax/Bcl-2 ratio, proteolytic activation of caspase-9 and caspase-3, and the degradation of poly(ADP-ribose) polymerase (PARP) protein. The effects of supercritical $CO_2$ extract from Phalsak on anoikis-resistant breast cancer stem cells suggest the use of this extract as a potential therapeutic agent for breast cancer stem cell treatment.

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