지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 201 - 208 (8page)
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초록· 키워드
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Beauveria bassiana is an efficient entomopathogenic fungus for biological control. Optimization of medium composition for production of B. bassiana BNBCRC spores in decanter cake (DC)-based solid culture was performed using response surface methodology (RSM). Effects of inorganic and organic nitrogen sources, mineral salt, and incubation temperature in a DC-based composition were experimentally studied in two stages, first using a Taguchi design. Among the decision variables tested, urea, peptone, and $MgSO_4$ significantly affected the spore concentration, and the maximal $1.89{\times}10^8\;spores\;g^{-1}$ was found at 30oC incubation temperature. In the second stage, these three selected decision variables were optimized by RSM, using a factorial central composite design. The maximum average spore concentration of B. bassiana BNBCRC increased to $4.80{\times}10^8\;spores\;g^{-1}$ in the optimal DC-based medium containing $0.80g\;L^{-1}$ urea, $2.10g\;L^{-1}$ peptone, and $0.75g\;L^{-1}$ $MgSO_4$. The coefficient of determination was $R^2=0.97$ for the RSM model fit to data. Compared to the original DC-based medium, the performed optimization increased the spore production of B. bassiana BNBCRC by 2.5 fold. After optimization of moisture content, the DC -based medium containing 60% moisture gave the highest spore concentration of $4.71{\times}10^8\;spores\;g^{-1}$.
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