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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 204 - 209 (6page)
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Osteoprotegerin (OPG) is a secreted glycoprotein and a member of the tumor necrosis factor receptor superfamily. It usually functions in bone remodeling, by inhibiting osteoclastogenesis through interaction with a receptor activator of the nuclear factor ${\kappa}B$ (RANKL). Transglutaminases-2 (Tgase-2) is a group of multifunctional enzymes that plays a role in cancer cell metastasis and bone formation. However, relationship between OPG and Tgase-2 is not studied. Therefore, we investigated the involvement of 12-O-Tetradecanoylphorbol 13-acetate in the expression of OPG in MG-63 osteosarcoma cells. Interleukin-$1{\beta}$ time-dependently induced OPG and Tgase-2 expression in cell lysates and media of the MG-63 cells by a Western blot. Additional 110 kda band was found in the media of MG-63 cells. 12-O-Tetradecanoylphorbol 13-acetate also induced OPG and Tgase-2 expression. However, an 110 kda band was not found in TPA-treated media of MG-63 cells. Cystamine, a Tgase-2 inhibitor, dose-dependently suppressed the expression of OPG in MG-63 cells. Gene silencing of Tgase-2 also significantly suppressed the expression of OPG in MG-63 cells. Next, we examined whether a band of 110 kda of OPG contains an isopeptide bond, an indication of Tgase-2 action, by monoclonal antibody specific for the isopeptide bond. However, we could not find the isopeptide bond at 110 kda but 77 kda, which is believed to be the band position of Tgase-2. This suggested that 110 kda is not the direct product of Tgase-2's action. All together, OPG and Tgase-2 is induced by IL-$1{\beta}$ or TPA in MG-63 cells and Tgase-2 is involved in OPG expression in MG-63 cells.
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참고문헌 (37)
참고문헌 신청
Augoulea, A. / 2013 / Osteoprotegerin as a marker of ath-erosclerosis in diabetic patients / Int. J. Endocrinol 2013:182060
Brandstrom, H. / 1998 / Tumor necrosis factor-alpha and -beta upregulate the levels of osteoprotegerin mRNA in human osteosarcoma MG-63 cells / Biochem. Biophys. Res. Commun 248:454~457
Cross, S. S. / 2006 / Osteoprotegerin (OPG)--a potential new role in the regulation of endothelial cell phenotype and tumour angiogenesis? / Int. J. Cancer 118:1901~1908
De Toni, E. N. / 2008 / OPG is regulated by beta-catenin and mediates resistance to TRAIL-induced apoptosis in colon cancer / Clin. Cancer Res 14:4713~4718
Emery, J. G. / 1998 / Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL / J. Biol. Chem 273:14363~14367
Brandstrom, H. / 1998 / Tumor necrosis factor-alpha and -beta upregulate the levels of osteoprotegerin mRNA in human osteosarcoma MG-63 cells / Biochem. Biophys. Res. Commun 248:454~457
Cross, S. S. / 2006 / Osteoprotegerin (OPG)--a potential new role in the regulation of endothelial cell phenotype and tumour angiogenesis? / Int. J. Cancer 118:1901~1908
De Toni, E. N. / 2008 / OPG is regulated by beta-catenin and mediates resistance to TRAIL-induced apoptosis in colon cancer / Clin. Cancer Res 14:4713~4718
Emery, J. G. / 1998 / Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL / J. Biol. Chem 273:14363~14367
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