지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 295 - 300 (6page)
이용수
초록· 키워드
오류제보하기
The actin cytoskeleton plays an important role in macrophage-mediated inflammatory responses by modulating the activation of Src and subsequently inducing nuclear factor (NF)-${\kappa}B$ translocation. In spite of its critical functions, few papers have examined how the actin cytoskeleton can be regulated by the activation of toll-like receptor (TLR). Therefore, in this study, we further characterized the biological value of the actin cytoskeleton in the functional activation of macrophages using an actin cytoskeleton disruptor, cytochalasin B (Cyto B), and explored the actin cytoskeleton's involvement in morphological changes, cellular attachment, and signaling events. Cyto B strongly suppressed the TLR4-mediated mRNA expression of inflammatory genes such as cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-${\alpha}$, and inducible nitric oxide (iNOS), without altering cell viability. This compound also strongly suppressed the morphological changes induced by lipopolysaccharide (LPS), a TLR4 ligand. Cyto B also remarkably suppressed NO production under non-adherent conditions but not in an adherent environment. Cyto B did not block the co-localization between surface glycoprotein myeloid differentiation protein-2 (MD2), a LPS signaling glycoprotein, and the actin cytoskeleton under LPS conditions. Interestingly, Cyto B and PP2, a Src inhibitor, enhanced the phagocytic uptake of fluorescein isothiocyanate (FITC)-dextran. Finally, it was found that Cyto B blocked the phosphorylation of vasodilator-stimulated phosphoprotein (VASP) at 1 min and the phosphorylation of heat shock protein 27 (HSP27) at 5 min. Therefore, our data suggest that the actin cytoskeleton may be one of the key components involved in the control of TLR4-mediated inflammatory responses in macrophages.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Coppolino, M. G / 2001 / Evidence fora molecular complex consisting of Fyb/SLAP, SLP-76, Nck, VASPand WASP that links the actin cytoskeleton to Fcgamma receptorsignalling during phagocytosis / J. Cell Sci 114:4307~4318
Diesel, B / 2013 / Activation of Rac1 GTPase by nanoparticulate structures inhuman macrophages / Eur. J. Pharm. Biopharm 84:315~324
Disanza, A / 2005 / Actin polymerization machinery: the fi nish line of signalingnetworks, the starting point of cellular movement / Cell. Mol. LifeSci 62:955~970
Duperrier, K / 2000 / Distinct subsets of dendritic cells resembling dermal DCs can begenerated in vitro from monocytes, in the presence of different serumsupplements / J. Immunol. Methods 238:119~131
Frleta, D / 2003 / CD40-mediated upregulationof Toll-like receptor 4-MD2 complex on the surface ofmurine dendritic cells / J. Leukoc. Biol 74:1064~1073
Diesel, B / 2013 / Activation of Rac1 GTPase by nanoparticulate structures inhuman macrophages / Eur. J. Pharm. Biopharm 84:315~324
Disanza, A / 2005 / Actin polymerization machinery: the fi nish line of signalingnetworks, the starting point of cellular movement / Cell. Mol. LifeSci 62:955~970
Duperrier, K / 2000 / Distinct subsets of dendritic cells resembling dermal DCs can begenerated in vitro from monocytes, in the presence of different serumsupplements / J. Immunol. Methods 238:119~131
Frleta, D / 2003 / CD40-mediated upregulationof Toll-like receptor 4-MD2 complex on the surface ofmurine dendritic cells / J. Leukoc. Biol 74:1064~1073
이 논문의 저자 정보
최근 본 자료
댓글(0)
0