지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 21 - 28 (8page)
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Microglia play a role in maintaining and resolving brain tissue homeostasis. In pathological conditions, microglia release pro-inflammatory cytokines and cytotoxic factors, which aggravate the progression of neurodegenerative diseases. Autophagy pathway might be involved in the production of pro-inflammatory cytokines and cytotoxic factors in microglia, though details of the mechanism remain largely unknown. In the present study, we examined the role of the autophagy pathway in activated BV2 microglia cells. In BV2 cells, rapamycin treatment activated the formation of anti-LC3-labeled autophagosomes, whereas the ATG5 depletion using siRNA-ATG5 prevented the formation of LC3-labeled autophagosomes, indicating that BV2 cells exhibit an active classical autophagy system. When treated with LPS, BV2 cells expressed an increase of anti-LC3-labeled dots. The levels of LC3-labeled dots were not suppressed, instead tended to be enhanced, by the inhibition of the autophagy pathway with siRNA-ATG5 or wortmannin, suggesting that LPS-induced LC3-labeled dots in nature were distinct from the typical autophagosomes. The levels of LPS-induced expression of iNOS and IL6 were suppressed by treatment with rapamycin, and conversely, their expressions were enhanced by siRNA-ATG5 treatment. Moreover, the activation of the autophagy pathway using rapamycin inhibited cell death of LPS-stimulated microglia. These results suggest that although microglia possess a typical autophagy pathway, the glial cells express a non-typical autophagy pathway in response to LPS, and the activation of the autophagy pathway suppresses the expression of iNOS and IL6, and the cell death of LPS-stimulated microglia.
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참고문헌 (49)
참고문헌 신청
Akar, U. / 2008 / Silencing of Bcl-2 expression by small interfering RNA induces autophagic cell death in MCF-7 breast cancer cells / Autophagy 4:669~679
Akiyama, H. / 2000 / Inflammation and Alzheimer's disease / Neurobiol. Aging 2:383~421
Alirezaei, M. / 2008 / Decreased neuronal autophagy in HIV dementia: a mechanism of indirect neurotoxicity / Autophagy 4:963~966
Beurel, E. / 2009 / Lipopolysaccharide-induced interleukin-6 production is controlled by glycogen synthase kinase-3 and STAT3 in the brain / J. Neuroinflammation 6:9
Canadien, V. / 2005 / Cutting edge: microbial products elicit formation of dendritic cell aggresome-like induced structures in macrophages / J. Immunol 174:2471~2475
Akiyama, H. / 2000 / Inflammation and Alzheimer's disease / Neurobiol. Aging 2:383~421
Alirezaei, M. / 2008 / Decreased neuronal autophagy in HIV dementia: a mechanism of indirect neurotoxicity / Autophagy 4:963~966
Beurel, E. / 2009 / Lipopolysaccharide-induced interleukin-6 production is controlled by glycogen synthase kinase-3 and STAT3 in the brain / J. Neuroinflammation 6:9
Canadien, V. / 2005 / Cutting edge: microbial products elicit formation of dendritic cell aggresome-like induced structures in macrophages / J. Immunol 174:2471~2475
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