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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 570 - 575 (6page)
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초록· 키워드
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This study was conducted to compare the effects of two forms of radiation (electron and X-ray; generated by an electron beam accelerator) on the conformation and antigenic properties of hen's egg albumin, ovalbumin (OVA), which was used as a model protein. OVA solutions (2.0 mg/mL) were individually irradiated by electron beam or X-ray at the absorbed doses of 0 (control), 2, 4, 6, 8, and 10 kGy. No differences between the two forms of radiation on the structural properties of OVA were shown by spectrometric and electrophoretic analyses. The turbidity of OVA solution increased and the main OVA bands on polyacrylamide gels disappeared after irradiation, regardless of the radiation source. In competitive indirect enzyme-linked immunosorbent assay, OVA samples irradiated by electron beam or X-ray showed different immunological responses in reactions with monoclonal and polyclonal antibodies (immunoglobulin G) produced against non-irradiated OVA. The results indicate that electron beam irradiation and X-ray irradiation produced different patterns of structural changes to the OVA molecule.
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참고문헌 (25)
참고문헌 신청
Al-kahtani, H. A / 1998 / Amino acid and protein changes in tilapia and Spanish mackerel after irradiation and storage / Radiat. Phys. Chem 51:107~114
Byun, M. W / 2000 / Effects of gamma irradiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp / J. Food Prot 63:940~944
Byun, M. W / 2009 / Information channel effects on women intention to purchase irradiated food in Korea / Radiat. Phys. Chem 78:675~677
Chan, J. K / 1993 / Thermal aggregation of myosin subfragments from cod and herring / J. Food Sci 58:1057~1061
Cho, K. H / 2001 / Changes of binding ability of milk-hypersensitive patients’ IgE to gamma-irradiated milk proteins / J. Korean Soc. Food Sci. Nutr 30:505~509
Byun, M. W / 2000 / Effects of gamma irradiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp / J. Food Prot 63:940~944
Byun, M. W / 2009 / Information channel effects on women intention to purchase irradiated food in Korea / Radiat. Phys. Chem 78:675~677
Chan, J. K / 1993 / Thermal aggregation of myosin subfragments from cod and herring / J. Food Sci 58:1057~1061
Cho, K. H / 2001 / Changes of binding ability of milk-hypersensitive patients’ IgE to gamma-irradiated milk proteins / J. Korean Soc. Food Sci. Nutr 30:505~509
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