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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 127 - 133 (7page)
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The Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) is one of the major genomic resources for human genetics and immunological studies. Use of LCLs is currently extended to pharmacogenetic studies to investigate variations in human gene expression as well as drug responses between individuals. We evaluated four common internal controls for gene expression analysis of selected hematopoietic transcriptional regulatory genes between B cells and LCLs. In this study, the expression pattern analyses showed that TBP (TATA box-binding protein) is a suitable internal control for normalization, whereas GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is not a good internal control for gene expression analyses of hematopoiesis-related genes between B cells and LCLs at different subculture passages. Using the TBP normalizer, we found significant gene expression changes in selected hematopoietic transcriptional regulatory genes (downregulation of RUNX1, RUNX3, CBFB, TLE1, and NOTCH2 ; upregulation of MSC and PLAGL2) between B cells and LCLs at different passage numbers. These results suggest that these hematopoietic transcriptional regulatory genes are potential cellular targets of EBV infection, contributing to EBV-mediated B-cell transformation and LCL immortalization.
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참고문헌 (30)
참고문헌 신청
Baik, S.Y. / 2007 / Identification of stathmin 1 expression induced by Epstein-Barr virus in human B lymphocytes / Cell Prolif 40:268~281
Baran-Marszak, F / 2002 / Gene array identification of Epstein Barr virus- regulated cellular genes in EBV-converted Burkitt lymphoma cell lines / Lab. Invest 82:1463~1479
Cahir-McFarland, E.D / 2004 / Role of NF-kappa B in cell survival and transcription of latent membrane protein 1-expressing or Epstein-Barr virus latency III-infected cells / J. Virol 78:4108~4119
Carter, K.L. / 2002 / Epstein-barr virus-induced changes in B-lymphocyte gene expression / J. Virol 76:10427~10436
de Kok, J.B / 2005 / Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes / Lab. Invest 85:154~159
Baran-Marszak, F / 2002 / Gene array identification of Epstein Barr virus- regulated cellular genes in EBV-converted Burkitt lymphoma cell lines / Lab. Invest 82:1463~1479
Cahir-McFarland, E.D / 2004 / Role of NF-kappa B in cell survival and transcription of latent membrane protein 1-expressing or Epstein-Barr virus latency III-infected cells / J. Virol 78:4108~4119
Carter, K.L. / 2002 / Epstein-barr virus-induced changes in B-lymphocyte gene expression / J. Virol 76:10427~10436
de Kok, J.B / 2005 / Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes / Lab. Invest 85:154~159
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