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논문 기본 정보

자료유형
학술저널
저자정보
Oh, Soojung (School of Mechanical and Aerospace Engineering, Seoul National University) Kim, Jangho (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) Ryu, Hyun Ryul (School of Mechanical and Aerospace Engineering, Seoul National University) Lim, Ki-Taek (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) Chung, Jong Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) Jeon, Noo Li (School of Mechanical and Aerospace Engineering, Seoul National University)
저널정보
한국농업기계학회 바이오시스템공학(구 한국농업기계학회지) 바이오시스템공학 제39권 제3호
발행연도
2014.1
수록면
244 - 252 (9page)

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Purpose: The development of an efficient in vitro cell culture device to process various cells would represent a major milestone in biological science and engineering. However, the current conventional macro-scale in vitro cell culture platforms are limited in their capacity for detailed analysis and determination of cellular behavior in complex environments. This paper describes a microfluidic-based culture device that allows accurate control of parameters of physical cues such as pressure. Methods: A microfluidic device, as a model microbioreactor, was designed and fabricated to culture Saccharomyces cerevisiae and Chlamydomonas reinhardtii under various conditions of physical pressure stimulus. This device was compatible with live-cell imaging and allowed quantitative analysis of physical cue-induced behavior in yeast and microalgae. Results: A simple microfluidic-based in vitro cell culture device containing a cell culture channel and an air channel was developed to investigate physical pressure stress-induced behavior in yeasts and microalgae. The shapes of Saccharomyces cerevisiae and Chlamydomonas reinhardtii could be controlled under compressive stress. The lipid production by Chlamydomonas reinhardtii was significantly enhanced by compressive stress in the microfluidic device when compared to cells cultured without compressive stress. Conclusions: This microfluidic-based in vitro cell culture device can be used as a tool for quantitative analysis of cellular behavior under complex physical and chemical conditions.

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