지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Objectives : ROS are involved in a wide spectrum of diseases including chronic inflammation and cancer. S.chinensis Baill, a perennial herb commonly called Chinese lizard's tail or Sam-baek-cho in Korea, is used for the treatment of edema and inflammatory diseases in the Oriental folk medicine. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the two extracts, water(WE) and ethyl acetate(EAE) from S.chinensis Baill. Methods : Anti-oxidant activity was evaluated using Fe2+ chelating and hydroxyl radical scavenging assay. DNA cleavage assay, and western blot and immunostaining for phospho-p65 were performed to evaluate anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell. Results : In Fe2+ chelating activity and hydroxyl radical scavenging activity, WE showed more strong scavenging activity for hydroxyl radical than EAE. WE scavenged hydroxyl radical by 12% at 3.2 ${\mu}g/ml$, 21% at 16 ${\mu}g/ml$, 32% at 80 ${\mu}g/ml$, 66% at 400 ${\mu}g/ml$ and 82% at 2000 ${\mu}g/ml$, respectively. In addition, WE showed more strong chelating activity than EAE. WE chelated Fe2+ ion by 1.1% at 3.2 ${\mu}g/ml$, 8.2% at 16 ${\mu}g/ml$, 26.3% at 80 ${\mu}g/ml$, 72% at 400 ${\mu}g/ml$ and 89% at 2000 ${\mu}g/ml$, respectively. Also, WE inhibited oxidative damage via its anti-oxidant activity. In anti-inflammatory effect, EAE inhibited NO production and iNOS expression. In addition EAE suppressed the NF-${\kappa}B$ and MAPK signaling pathway in LPS-stimulated RAW 264.7 cells. Conclusions : Together, these data indicate that S. chinensis Baill, shows anti-oxidant activity and anti-inflammatory effect.
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참고문헌 (15)
참고문헌 신청
Durackova Z. / 2010 / Some current insights into oxidative stress / Physiol Res 59:459~469
Fang J. / 2009 / Therapeutic strategies by modulating oxygen stress in cancer and inflammation / Adv Drug Deliv Rev 61:290~302
Khandrika L. / 2009 / Oxidative stress in prostate cancer / Cancer Lett 282:125~136
Reuter S. / 2010 / Oxidative stress, inflammation, and cancer : How are they linked / Free Radic Biol Med 11:1603~1616
Gutteridge JM. / 1995 / Lipid peroxidation and antioxidants as biomarkers of tissue damage / Clin Chem 41:1819~1828
Fang J. / 2009 / Therapeutic strategies by modulating oxygen stress in cancer and inflammation / Adv Drug Deliv Rev 61:290~302
Khandrika L. / 2009 / Oxidative stress in prostate cancer / Cancer Lett 282:125~136
Reuter S. / 2010 / Oxidative stress, inflammation, and cancer : How are they linked / Free Radic Biol Med 11:1603~1616
Gutteridge JM. / 1995 / Lipid peroxidation and antioxidants as biomarkers of tissue damage / Clin Chem 41:1819~1828
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