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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Su-Jeong (Institute of Medical Science, Yeungnam University) Gu, Hee-Jin (Clinical Trial Center for Medical Devices of Yeungnam University Hospital) Cho, Yun-Woo (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) Park, Hea-Woon (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu) Lee, Joon-Ha (Department of Biochemistry and Molecular Biology, Yeungnam University) Hwang, Se-Jin (Department of Anatomy and Cell Biology, College of Medicine, Hanyang University) Ahn, Sang-Ho (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University)
저널정보
대한물리치료학회 대한물리치료학회지(JKPT) 대한물리치료학회지 제22권 제3호
발행연도
2010.1
수록면
93 - 98 (6page)

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Purpose: To investigate temporal changes in IL-1${\beta}$ mRNA expression in spinal dorsal horn (DH) and dorsal root ganglion (DRG) in a rat lumbar disc herniation (LDH) model. Methods: Autologous nucleus pulposus, harvested from the tail disc between the second and third coccygeal vertebrae (Co2-3), was implanted next to the left L5 nerve root just proximal to the DRG after partial laminectomy. IL-1${\beta}$ mRNA expression was investigated in DRG and DH in our LDH model. Real-time PCR assays were done using a 7500 Real Time PCR system (Applied Biosystems, USA). Results: Expression of IL-1${\beta}$ in DRG and DH was observed for 30 days postoperatively. Expression of IL-1${\beta}$ mRNA in the ipsilateral DRG of the LDH group gradually increased from 5 to 30 days after surgery. The amount of IL-1${\beta}$ in the contralateral DRG peaked 10 days after surgery and then gradually decreased. However, there was no difference in IL-1${\beta}$ mRNA expression in spinal DH between the LDH group and the sham-operated group. Conclusion: Long-term expression of IL-1${\beta}$ in the LDH model may worsen the chronic pain state. Future studies on inhibition of IL-1${\beta}$ expression in the LDH model will be needed to develop selective treatment strategies for patients with LDH.

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