Lack of Association of Intron 3 16 bp Polymorphism of TP53 with Breast Cancer among Iranian-Azeri Patients

Pouladi, Nasser; Kouhsari, Shideh Montasser; Feizi, Mohammadali Hosseinpour; Dehghan, Roghayeh; Azarfam, Parvin; Farajzadeh, Davoud

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자료유형: 학술저널

저자정보: Pouladi, Nasser (Department of Cellular and Molecular Biology, School of Biology, College of Sciences, University of Tehran) Kouhsari, Shideh Montasser (Department of Cellular and Molecular Biology, School of Biology, College of Sciences, University of Tehran) Feizi, Mohammadali Hosseinpour (Department of Biology, Faculty of Natural Science, University of Tabriz) Dehghan, Roghayeh (Department of Biology, Faculty of Natural Science, University of Tabriz) Azarfam, Parvin (Department of Biology, Faculty of Natural Science, University of Tabriz) Farajzadeh, Davoud (Department of Biology, Faculty of Science, Azarbaijan Shahid Madani University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제6호

발행연도: 2014.1

수록면: 2,631 - 2,634 (4page)

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Background: p53 gene is a well-known tumor suppressor gene that has several polymorphisms in both its exons and introns. It has been suggested that intron 3 16 bp duplication polymorphism may affect the gene function resulting in reduction or suppression of p53 anti tumor activity. In most case control studies a duplicated allele has been noticeably more frequent in cases rather than controls but there are also conflicting results. The aim of this study was to assess the association of intron 3 16 bp duplication polymorphism of p53 with breast cancer risk among Iranian-Azeri population. We also analyzed the clinicopathological information of patients as an epidemiological description of breast cancer in the north-west of Iran. Materials and Methods: This case-control study was performed on 221 breast cancer patients and 170 controls. Genomic DNA was extracted from peripheral blood samples and tumor tissues. p53 PIN3 genotype was determined using electrophoresis of PCR products on 8% non-denaturing polyacrylamide gels and silver staining. Results: In the control and case groups, respectively, 62.9% and 61.1% had no 16 bp insertion (A1A1 genotype), 7.1% and 7.7% had insertion in both p53 alleles (A2A2) and 30% and 31.2% were heterozygous (A1A2). There was no significant difference between genotype frequencies as well as allelic frequencies in two case and control groups. Conclusions: According to the result of the present study, the intron 3 16 bp duplication polymorphism of p53 could not be assessed as a marker of risk factor for predisposition to breast cancer in Azeri population. However, a high frequency of A2 allele (22.1%) in our population suggested that intron 3 16 bp duplication polymorphism may be a valuable marker for study in other cancers with well designed large groups.

#Intron 3 #p53 gene #polymorphism #breast cancer

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