Epigallocatechin-3-gallate Inhibits Tax-dependent Activation of Nuclear Factor Kappa B and of Matrix Metalloproteinase 9 in Human T-cell Lymphotropic Virus-1 Positive Leukemia Cells

Harakeh, Steve; Diab-Assaf, Mona; Azar, Rania; Hassan, Hani Mutlak Abdulla; Tayeb, Safwan; Abou-El-Ardat, Khalil; Damanhouri, Ghazi Abdullah; Qadri, Ishtiaq; Abuzenadah, Adel; Chaudhary, Adeel; Kumosani, Taha; Niedzwiecki, Aleksandra; Rath, Mathias; Yacoub, Haitham; Azhar, Esam; Barbour, Elie

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자료유형: 학술저널

저자정보: Harakeh, Steve (Special Infectious Agents Unit, King Abdulaziz University) Diab-Assaf, Mona (Lebanese University, EDST ["Molecular Tumor genesis and Anticancer Pharmacology"]) Azar, Rania (Lebanese University, EDST ["Molecular Tumor genesis and Anticancer Pharmacology"]) Hassan, Hani Mutlak Abdulla (King Fahd Medical Research Center, King Abdulaziz University) Tayeb, Safwan (Special Infectious Agents Unit, King Abdulaziz University) Abou-El-Ardat, Khalil (Biology Department, American University of Beirut) Damanhouri, Ghazi Abdullah (King Fahd Medical Research Center, King Abdulaziz University) Qadri, Ishtiaq (King Fahd Medical Research Center, King Abdulaziz University) Abuzenadah, Adel (King Fahd Medical Research Center, King Abdulaziz University) Chaudhary, Adeel (King Fahd Medical Research Center, King Abdulaziz University) Kumosani, Taha (King Fahd Medical Research Center, King Abdulaziz University) Niedzwiecki, Aleksandra (Dr. Rath Research Institute) Rath, Mathias (Dr. Rath Research Institute) Yacoub, Haitham (King Fahd Medical Research Center, King Abdulaziz University) Azhar, Esam (Special Infectious Agents Unit, King Abdulaziz University) Barbour, Elie (Department of Animal and Veterinary Sciences, American University of Beirut)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제3호

발행연도: 2014.1

수록면: 1,219 - 1,225 (7page)

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Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol molecule from green tea and is known to exhibit antioxidative as well as tumor suppressing activity. In order to examine EGCG tumor invasion and suppressing activity against adult T-cell leukemia (ATL), two HTLV-1 positive leukemia cells (HuT-102 and C91-PL) were treated with non-cytotoxic concentrations of EGCG for 2 and 4 days. Proliferation was significantly inhibited by 100 ${\mu}M$ at 4 days, with low cell lysis or cytotoxicity. HTLV-1 oncoprotein (Tax) expression in HuT-102 and C91-PL cells was inhibited by 25 ${\mu}M$ and 125 ${\mu}M$ respectively. The same concentrations of EGCG inhibited NF-kB nuclearization and stimulation of matrix metalloproteinase-9 (MMP-9) expression in both cell lines. These results indicate that EGCG can inhibit proliferation and reduce the invasive potential of HTLV-1-positive leukemia cells. It apparently exerted its effects by suppressing Tax expression, manifested by inhibiting the activation of NF-kB pathway and induction of MMP-9 transcription in HTLV-1 positive cells.

#Acute T-cell leukemia #human T-cell lymphotropic virus type I #HTLV-1) #Tax #NF-kB pathway

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