Differential Protein Expression Profile Between CD20 Positive and Negative Cells of the NCI-H929 Cell Line

Geng, Chuan-Ying; Liu, Nian; Yang, Guang-Zhong; Liu, Ai-Jun; Leng, Yun; Wang, Hui-Juan; Li, Li-Hong; Wu, Yin; Li, Yan-Chen; Chen, Wen-Ming

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자료유형: 학술저널

저자정보: Geng, Chuan-Ying (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Liu, Nian (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Yang, Guang-Zhong (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Liu, Ai-Jun (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Leng, Yun (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Wang, Hui-Juan (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Li, Li-Hong (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Wu, Yin (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Li, Yan-Chen (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University) Chen, Wen-Ming (Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제13권 제11호

발행연도: 2012.1

수록면: 5,409 - 5,413 (5page)

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At present, multiple myeloma (MM) remains an incurable disease and cologenic cells may be responsible for disease relapse. It has been proposed that CD20+/CD138- NCI-H929 cells could be hallmarks of MM clonogenic cells. Here, the immunology phenotype of NCI-H929 cells is described. Only a small population of CD20+/CD138- cells (<1%) was found in the NCI-H929 cell line, but CD20+/CD138- cells were not detected. We found that CD20+/CD138+ cells were able to exhibit cologenic capacity by colony formation assay and continuous passage culture. Proteins were analyzed by 1D-SDS-PAGE and TMT based quantitative differential liquid chromatography tandem mass spectrometry (LC-MS/MS). 1,082 non-redundant proteins were identified, 658 of which were differentially expressed with at least a 1.5-fold difference. 205 proteins in CD20+ cells were expressed at higher levels and 453 proteins were at lower levels compared with CD20- cells. Most proteins had catalytic and binding activity and mainly participated in metabolic processes, cell communication and molecular transport. These results proved that there are different biological features and protein expression profile between CD20+ and CD20- cells in the NCI-H929 cell line.

#Multiple myeloma #NCI-H929 cells #proteomics #mass spectrometry

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